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Sensors 2018, 18(2), 589; doi:10.3390/s18020589 (registering DOI)

Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers

1
Department of Food and Environmental Engineering, Vocational and Technical College, Chuzhou 239001, China
2
Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China
*
Authors to whom correspondence should be addressed.
Received: 29 November 2017 / Revised: 5 February 2018 / Accepted: 6 February 2018 / Published: 14 February 2018
(This article belongs to the Special Issue Semiconductor Materials on Biosensors Application)
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Abstract

Carboxyl-functionalized semiconducting polymer dots (Pdots) were synthesized as an energy donor by the nanoprecipitation method. A black hole quenching dye (BHQ-labelled thrombin aptamers) was used as the energy acceptor, and fluorescence resonance energy transfer between the aptamers and Pdots was used for fluorescence quenching of the Pdots. The addition of thrombin restored the fluorescence intensity. Under the optimized experimental conditions, the fluorescence of the system was restored to the maximum when the concentration of thrombin reached 130 nM, with a linear range of 0–50 nM (R2 = 0.990) and a detection limit of 0.33 nM. This sensor was less disturbed by impurities, showing good specificity and signal response to thrombin, with good application in actual samples. The detection of human serum showed good linearity in the range of 0–30 nM (R2 = 0.997), with a detection limit of 0.56 nM and a recovery rate of 96.2–104.1%, indicating that this fluorescence sensor can be used for the detection of thrombin content in human serum. View Full-Text
Keywords: semiconducting polymer dots; thrombin; aptamer; fluorescence resonance energy trans semiconducting polymer dots; thrombin; aptamer; fluorescence resonance energy trans
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MDPI and ACS Style

Liu, Y.; Jiang, X.; Cao, W.; Sun, J.; Gao, F. Detection of Thrombin Based on Fluorescence Energy Transfer between Semiconducting Polymer Dots and BHQ-Labelled Aptamers. Sensors 2018, 18, 589.

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