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Sensors 2017, 17(9), 1954; https://doi.org/10.3390/s17091954

A Simple and Selective Fluorescent Sensor Chip for Indole-3-Butyric Acid in Mung Bean Sprouts Based on Molecularly Imprinted Polymer Coatings

1
Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education & Xinjiang Uygur Autonomous Region, College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, China
2
Université de Bordeaux, IMS, Institut Polytechnique de Bordeaux, CNRS, UMR 5218, F-33607 Pessac CEDEX, France
*
Author to whom correspondence should be addressed.
Received: 26 July 2017 / Revised: 15 August 2017 / Accepted: 22 August 2017 / Published: 24 August 2017
(This article belongs to the Special Issue Novel Approaches to Biosensing with Nanoparticles)
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Abstract

In this paper, we report the preparation of molecularly imprinted polymer coatings on quartz chips for selective solid-phase microextraction and fluorescence sensing of the auxin, indole-3-butyric acid. The multiple copolymerization method was used to prepare polymer coatings on silylated quartz chips. The polymer preparation conditions (e.g., the solvent, monomer, and cross-linker) were investigated systemically to enhance the binding performance of the imprinted coatings. Direct solid-phase fluorescence measurements on the chips facilitated monitoring changes in coating performance. The average binding capacity of an imprinted polymer coated chip was approximately 152.9 µg, which was higher than that of a non-imprinted polymer coated chip (60.8 µg); the imprinted coatings showed the highest binding to IBA among the structural analogues, indicating that the coatings possess high selectivity toward the template molecule. The developed method was used for the determination of the auxin in mung bean extraction, and the recovery was found to be in the range of 91.5% to 97.5%, with an RSD (n = 3) of less than 7.4%. Thus, the present study provides a simple method for fabricating a fluorescent sensor chip for selective analysis. View Full-Text
Keywords: indole-3-butyric acid; polymer coating; molecularly imprinted polymer; fluorescence detection; molecularly imprinted polymer; sensor chip indole-3-butyric acid; polymer coating; molecularly imprinted polymer; fluorescence detection; molecularly imprinted polymer; sensor chip
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chang, J.; Bahethan, B.; Muhammad, T.; Yakup, B.; Abbas, M. A Simple and Selective Fluorescent Sensor Chip for Indole-3-Butyric Acid in Mung Bean Sprouts Based on Molecularly Imprinted Polymer Coatings. Sensors 2017, 17, 1954.

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