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Sensors 2017, 17(5), 1158; doi:10.3390/s17051158

Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

1
Centre for Biomedical Technology, Optics, Photonics and Biophotinics Laboratory, Campus Montegancedo, Universidad Politécnica de Madrid, 28223 Madrid, Spain
2
BioOptical Detection, Centro de Empresas, Campus Montegancedo, 28223 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Nicole Jaffrezic-Renault
Received: 11 April 2017 / Revised: 13 May 2017 / Accepted: 16 May 2017 / Published: 19 May 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1783 KB, uploaded 19 May 2017]   |  

Abstract

A novel compact optical biochip based on a thin layer-sensing surface of nitrocellulose is used for in-situ label-free detection of metalloproteinase (MMP9) related to dry eye disease. In this article, a new integrated chip with different interferometric transducers layout with an optimal sensing surface is reported for the first time. We demonstrate that specific antibodies can be immobilized onto these transducers with a very low volume of sample and with good orientation. Many sensing transducers constitute the presented biochip in order to yield statistical data and stability in the acquired measurements. As a result, we report the recognition curve for pure recombinant MMP9, tests of model tears with MMP9, and real tear performance from patients, with a promising limit of detection. View Full-Text
Keywords: nitrocellulose; biosensor; photonic; label-free; dry eye; MMP9; low detection; low volume; compact; biosurface nitrocellulose; biosensor; photonic; label-free; dry eye; MMP9; low detection; low volume; compact; biosurface
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Santamaría, B.; Laguna, M.F.; López-Romero, D.; Hernandez, A.L.; Sanza, F.J.; Lavín, Á.; Casquel, R.; Maigler, M.V.; Espinosa, R.L.; Holgado, M. Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis. Sensors 2017, 17, 1158.

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