Next Article in Journal
Low Duty-Cycling MAC Protocol for Low Data-Rate Medical Wireless Body Area Networks
Previous Article in Journal
A Robust Method to Detect BeiDou Navigation Satellite System Orbit Maneuvering/Anomalies and Its Applications to Precise Orbit Determination
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(5), 1132; doi:10.3390/s17051132

Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous

1
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia
2
Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Pilloton
Received: 7 March 2017 / Revised: 14 April 2017 / Accepted: 21 April 2017 / Published: 16 May 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [3524 KB, uploaded 16 May 2017]   |  

Abstract

In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable. View Full-Text
Keywords: tyrosinase; graphene-gold nanocomposite; electrochemical biosensor; phenolic compounds tyrosinase; graphene-gold nanocomposite; electrochemical biosensor; phenolic compounds
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Fartas, F.M.; Abdullah, J.; Yusof, N.A.; Sulaiman, Y.; Saiman, M.I. Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous. Sensors 2017, 17, 1132.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top