Quantitative Detection of NADH Using a Novel Enzyme-Assisted Method Based on Surface-Enhanced Raman Scattering
AbstractAn enzymatic method for quantitative detection of the reduced form of nicotinamide-adenine dinucleotide (NADH) using surface-enhanced Raman scattering was developed. Under the action of NADH oxidase and horseradish peroxidase, NADH can generate hydrogen peroxide (H2O2) in a 1:1 molar ratio, and the H2O2 can oxidize a chromogen into pigment with a 1:1 molar ratio. Therefore, the concentration of NADH can be determined by detecting the generated pigment. In our experiments, eight chromogens were studied, and o-tolidine (OT) was selected because of the unique Raman peaks displayed by its corresponding pigment. The optimal OT concentration was 2 × 10−3 M, and this gave the best linear relationship and the widest linear range between the logarithmic H2O2 concentration and the logarithmic integrated SERS intensity of the peak centered at 1448 cm−1. Under this condition, the limit of detection for NADH was as low as 4 × 10−7 M. Two NADH samples with concentrations of 2 × 10−4 and 2 × 10−5 M were used to validate the linear relationship, and the logarithmic deviations were less than 3%. View Full-Text
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Teng, H.; Lv, M.; Liu, L.; Zhang, X.; Zhao, Y.; Wu, Z.; Xu, H. Quantitative Detection of NADH Using a Novel Enzyme-Assisted Method Based on Surface-Enhanced Raman Scattering. Sensors 2017, 17, 788.
Teng H, Lv M, Liu L, Zhang X, Zhao Y, Wu Z, Xu H. Quantitative Detection of NADH Using a Novel Enzyme-Assisted Method Based on Surface-Enhanced Raman Scattering. Sensors. 2017; 17(4):788.Chicago/Turabian Style
Teng, Haiyan; Lv, Mingyang; Liu, Luo; Zhang, Xin; Zhao, Yongmei; Wu, Zhenglong; Xu, Haijun. 2017. "Quantitative Detection of NADH Using a Novel Enzyme-Assisted Method Based on Surface-Enhanced Raman Scattering." Sensors 17, no. 4: 788.
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