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Sensors 2017, 17(3), 480; doi:10.3390/s17030480

Rapid and Sensitive Lateral Flow Immunoassay Method for Procalcitonin (PCT) Based on Time-Resolved Immunochromatography

1
Institute of Antibody Engineering, State Key Laboratory of Organ Failure Research, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, China
2
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
3
R&D Center, DaRui Biotechnology Co., Ltd., Guangzhou 510655, China
*
Author to whom correspondence should be addressed.
Academic Editor: Nicole Jaffrezic-Renault
Received: 10 January 2017 / Revised: 24 February 2017 / Accepted: 24 February 2017 / Published: 28 February 2017
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1539 KB, uploaded 28 February 2017]   |  

Abstract

Procalcitonin (PCT) is a current, frequently-used marker for severe bacterial infection. The aim of this study was to develop a cost-effective detection kit for rapid quantitative and on-site detection of PCT. To develop the new PCT quantitative detecting kit, a double-antibody sandwich immunofluorescent assay was employed based on time-resolved immunofluorescent assay (TRFIA) combined with lateral flow immunoassay (LFIA). The performance of the new developed kit was evaluated in the aspects of linearity, precision, accuracy, and specificity. Two-hundred thirty-four serum samples were enrolled to carry out the comparison test. The new PCT quantitative detecting kit exhibited a higher sensitivity (0.08 ng/mL). The inter-assay coefficient of variation (CV) and the intra-assay CV were 5.4%–7.7% and 5.7%–13.4%, respectively. The recovery rates ranged from 93% to 105%. Furthermore, a high correlation (n = 234, r = 0.977, p < 0.0001) and consistency (Kappa = 0.875) were obtained when compared with the PCT kit from Roche Elecsys BRAHMS. Thus, the new quantitative method for detecting PCT has been successfully established. The results indicated that the newly-developed system based on TRFIA combined with LFIA was suitable for rapid and on-site detection for PCT, which might be a useful platform for other biomarkers in point-of-care tests. View Full-Text
Keywords: time-resolved; immunochromatography; procalcitonin (PCT) time-resolved; immunochromatography; procalcitonin (PCT)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shao, X.-Y.; Wang, C.-R.; Xie, C.-M.; Wang, X.-G.; Liang, R.-L.; Xu, W.-W. Rapid and Sensitive Lateral Flow Immunoassay Method for Procalcitonin (PCT) Based on Time-Resolved Immunochromatography. Sensors 2017, 17, 480.

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