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Sensors 2017, 17(3), 442; doi:10.3390/s17030442

Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

1
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
2
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Jean-Louis Marty, Silvana Andreescu and Akhtar Hayat
Received: 27 December 2016 / Revised: 15 February 2017 / Accepted: 21 February 2017 / Published: 23 February 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [5992 KB, uploaded 23 February 2017]   |  

Abstract

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens. View Full-Text
Keywords: food-borne pathogens; fluorescence; automated instrument system; on-site detection; quantum dots; LabVIEW food-borne pathogens; fluorescence; automated instrument system; on-site detection; quantum dots; LabVIEW
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lu, Z.; Zhang, J.; Xu, L.; Li, Y.; Chen, S.; Ye, Z.; Wang, J. Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens. Sensors 2017, 17, 442.

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