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Sensors 2017, 17(2), 292; doi:10.3390/s17020292

A Multichannel Calorimetric Simultaneous Assay Platform Using a Microampere Constant-Current Looped Enthalpy Sensor Array

1
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
2
Department of Biotechnology, Asia University, Taichung 41354, Taiwan
3
Education and Research Center for Bio-Industrial Automation, National Taiwan University, Taipei 10617, Taiwan
4
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40447, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Vittorio M. N. Passaro
Received: 30 October 2016 / Revised: 23 January 2017 / Accepted: 1 February 2017 / Published: 4 February 2017
(This article belongs to the Section Physical Sensors)
View Full-Text   |   Download PDF [2062 KB, uploaded 4 February 2017]   |  

Abstract

Calorimetric biochemical measurements offer various advantages such as low waste, low cost, low sample consumption, short operating time, and labor-savings. Multichannel calorimeters can enhance the possibility of performing higher-throughput biochemical measurements. An enthalpy sensor (ES) array is a key device in multichannel calorimeters. Most ES arrays use Wheatstone bridge amplifiers to condition the sensor signals, but such an approach is only suitable for null detection and low resistance sensors. To overcome these limitations, we have developed a multichannel calorimetric simultaneous assay (MCSA) platform. An adjustable microampere constant-current (AMCC) source was designed for exciting the ES array using a microampere current loop measurement circuit topology. The MCSA platform comprises a measurement unit, which contains a multichannel calorimeter and an automatic simultaneous injector, and a signal processing unit, which contains multiple ES signal conditioners and a data processor. This study focused on the construction of the MCSA platform; in particular, construction of the measurement circuit and calorimeter array in a single block. The performance of the platform, including current stability, temperature sensitivity and heat sensitivity, was evaluated. The sensor response time and calorimeter constants were given. The capability of the platform to detect relative enzyme activity was also demonstrated. The experimental results show that the proposed MCSA is a flexible and powerful biochemical measurement device with higher throughput than existing alternatives. View Full-Text
Keywords: multichannel calorimeter; enthalpy sensor array; adjustable microampere constant current loop; simultaneous assay; relative enzyme activity; catalase multichannel calorimeter; enthalpy sensor array; adjustable microampere constant current loop; simultaneous assay; relative enzyme activity; catalase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wei, H.-C.; Huang, S.-H.; Jiang, J.-A.; Lee, Y.-C. A Multichannel Calorimetric Simultaneous Assay Platform Using a Microampere Constant-Current Looped Enthalpy Sensor Array. Sensors 2017, 17, 292.

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