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Sensors 2017, 17(2), 281; doi:10.3390/s17020281

An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection

Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan
Author to whom correspondence should be addressed.
Received: 30 November 2016 / Accepted: 25 January 2017 / Published: 1 February 2017
(This article belongs to the Special Issue Gas Sensors for Health Care and Medical Applications)
View Full-Text   |   Download PDF [823 KB, uploaded 13 February 2017]   |  


This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases. View Full-Text
Keywords: electrochemical sensor; chromatography paper-based sensor; gas sensor electrochemical sensor; chromatography paper-based sensor; gas sensor

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kuretake, T.; Kawahara, S.; Motooka, M.; Uno, S. An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection. Sensors 2017, 17, 281.

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