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Sensors 2017, 17(11), 2594; doi:10.3390/s17112594

A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite

1,* , 1
,
1,†
and
2,†
1
Key Laboratory of Sensor, Beijing Information Science and Technology University, Beijing 100000, China
2
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Science, Beijing 100000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 September 2017 / Revised: 19 October 2017 / Accepted: 7 November 2017 / Published: 10 November 2017
(This article belongs to the Section Biosensors)
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Abstract

We have developed a novel mediated biochemical oxygen demand (BOD) biosensor based on immobilized Bacillus subtilis (B. subtilis) on three-dimensional (3D) porous graphene-polypyrrole (rGO-PPy) composite. The 3D porous rGO-PPy composite was prepared using hydrothermal method following with electropolymerization. Then the 3D porous rGO-PPy composite was used as a support for immobilizing negatively charged B. subtilis denoted as rGO-PPy-B through coordination and electrostatic interaction. Further, the prepared rGO-PPy-B was used as a microbial biofilm for establishing a mediated BOD biosensor with ferricyanide as an electronic acceptor. The indirect determination of BOD was performed by electrochemical measuring ferrocyanide generated from a reduced ferricyanide mediator using interdigited ultramicroelectrode array (IUDA) as the working electrode. The experimental results suggested a good linear relationship between the amperometric responses and BOD standard concentrations from 4 to 60 mg/L, with a limit detection of 1.8 mg/L (S/N ≥ 3). The electrochemical measurement of real water samples showed a good agreement with the conventional BOD5 method, and the good anti-interference as well as the long-term stability were well demonstrated, indicating that the proposed mediated BOD biosensor in this study holds a potential practical application of real water monitoring. View Full-Text
Keywords: mediated BOD biosensor; immobilized B. subtilis; 3D porous graphene-polypyrrole composite; electrochemical measurement mediated BOD biosensor; immobilized B. subtilis; 3D porous graphene-polypyrrole composite; electrochemical measurement
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hu, J.; Li, Y.; Gao, G.; Xia, S. A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite. Sensors 2017, 17, 2594.

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