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Sensors 2017, 17(10), 2363; doi:10.3390/s17102363

A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection

Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Helios #01-02, 11 Biopolis Way, Singapore 138667, Singapore
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Received: 30 August 2017 / Revised: 29 September 2017 / Accepted: 11 October 2017 / Published: 16 October 2017
(This article belongs to the Special Issue Surface Plasmon Resonance Sensing)
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Abstract

In this paper, we present a phase-intensity surface plasmon resonance (SPR) biosensor and demonstrate its use for avian influenza A (H5N1) antibody biomarker detection. The sensor probes the intensity variation produced by the steep phase response at surface plasmon excitation. The prism sensor head is fixed between a pair of polarizers with a perpendicular orientation angle and a forbidden transmission path. At SPR, a steep phase change is introduced between the p- and s-polarized light, and this rotates the polarization ellipse of the transmission beam. This allows the light at resonance to be transmitted and a corresponding intensity change to be detected. Neither time-consuming interference fringe analysis nor a phase extraction process is required. In refractive index sensing experiments, the sensor resolution was determined to be 6.3 × 10−6 refractive index values (RIU). The sensor has been further applied for H5N1 antibody biomarker detection, and the sensor resolution was determined to be 193.3 ng mL−1, compared to 1 μg mL−1 and 0.5 μg mL−1, as reported in literature for influenza antibody detection using commercial Biacore systems. It represents a 517.3% and 258.7% improvement in detection limit, respectively. With the unique features of label-free, real-time, and sensitive detection, the phase-intensity SPR biosensor has promising potential applications in influenza detection. View Full-Text
Keywords: SPR biosensor; surface plasmon resonance; phase-intensity detection; avian influenza A; H5N1 SPR biosensor; surface plasmon resonance; phase-intensity detection; avian influenza A; H5N1
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wong, C.L.; Chua, M.; Mittman, H.; Choo, L.X.; Lim, H.Q.; Olivo, M. A Phase-Intensity Surface Plasmon Resonance Biosensor for Avian Influenza A (H5N1) Detection. Sensors 2017, 17, 2363.

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