Next Article in Journal
A Low Power Consumption Algorithm for Efficient Energy Consumption in ZigBee Motes
Next Article in Special Issue
Microbial Fuels Cell-Based Biosensor for Toxicity Detection: A Review
Previous Article in Journal
American Sign Language Alphabet Recognition Using a Neuromorphic Sensor and an Artificial Neural Network
Previous Article in Special Issue
Sensitive Genotyping of Foodborne-Associated Human Noroviruses and Hepatitis A Virus Using an Array-Based Platform
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Sensors 2017, 17(10), 2178; doi:10.3390/s17102178

Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem

1
CMUA, Department of Electrical and Electronics Engineering, Universidad de los Andes, 111711 Bogota, Colombia
2
CorpoGen, Carrera 5 No. 66A-34, 110231 Bogota, Colombia
*
Author to whom correspondence should be addressed.
Received: 5 August 2017 / Revised: 2 September 2017 / Accepted: 7 September 2017 / Published: 22 September 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [5626 KB, uploaded 22 September 2017]   |  

Abstract

Bioelectrochemical sensing of Mycobacterium tuberculosis through electro-immunosensors is a promising technique to detect relevant analytes. In general, immunosensors require the formation of organic assemblies by the adsorption of molecular constituents. Moreover, they depend on the correct immobilization of the bio-recognition element in the biosensor. These procedures cannot be easily monitored without the use of invasive methods. In this work, an impedance analysis technique was used, as a non-invasive method, to measure and differentiate the manufacturing stages of the sensors. Biomicrosystems were fabricated through physical vapor deposition (PVD) of 80 nm Au nanolayers on 35 µm copper surfaces. Later, the surface was modified through thiolation methods generating a self-assembled-monolayer (SAM) with 20 mM 4-aminothiophenol (4-ATP) on which a polyclonal antibody (pAb) was covalently attached. Using impedance analysis, every step of the electro-immunosensor fabrication protocol was characterized using 40 independent replicas. Results showed that, compared to the negative controls, distilled water, and 0.5 µg/mL HSA, a maximum variation of 171% between each replica was achieved when compared to samples containing 0.5 µg/mL of ESAT-6 M. tuberculosis immunodominant protein. Therefore, this development validates a non-invasive method to electrically monitor the assembly process of electro-immunosensors and a tool for its further measure for detection of relevant antigens. View Full-Text
Keywords: Mycobacterium tuberculosis; ESAT-6; biosensor; impedance; biomicrosystem Mycobacterium tuberculosis; ESAT-6; biosensor; impedance; biomicrosystem
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sepulveda, D.; Aroca, M.A.; Varela, A.; Del Portillo, P.; Osma, J.F. Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem. Sensors 2017, 17, 2178.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top