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Sensors 2016, 16(9), 1365;

Patterned Array of Poly(ethylene glycol) Silane Monolayer for Label-Free Detection of Dengue

Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Author to whom correspondence should be addressed.
Academic Editors: Alexandre Francois and Al Meldrum
Received: 17 March 2016 / Revised: 4 July 2016 / Accepted: 6 July 2016 / Published: 25 August 2016
(This article belongs to the Special Issue Label-Free Optical Biosensors)
View Full-Text   |   Download PDF [3773 KB, uploaded 25 August 2016]   |  


In the present study, the construction of arrays on silicon for naked-eye detection of DNA dengue was demonstrated. The array was created by exposing a polyethylene glycol (PEG) silane monolayer to 254 nm ultraviolet (UV) light through a photomask. Formation of the PEG silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. The results of XPS confirmed that irradiation of ultraviolet (UV) light generates an aldehyde functional group that offers conjugation sites of amino DNA probe for detection of a specific dengue virus target DNA. Employing a gold enhancement process after inducing the electrostatic interaction between positively charged gold nanoparticles and the negatively charged target DNA hybridized to the DNA capture probe allowed to visualize the array with naked eye. The developed arrays demonstrated excellent performance in diagnosis of dengue with a detection limit as low as 10 pM. The selectivity of DNA arrays was also examined using a single base mismatch and noncomplementary target DNA. View Full-Text
Keywords: UV-arrays; PEG silane monolayer; DNA dengue; gold nanoparticles UV-arrays; PEG silane monolayer; DNA dengue; gold nanoparticles

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Rosly, N.Z.; Ahmad, S.A.A.; Abdullah, J.; Yusof, N.A. Patterned Array of Poly(ethylene glycol) Silane Monolayer for Label-Free Detection of Dengue. Sensors 2016, 16, 1365.

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