Next Article in Journal
Mathematical Model and Calibration Experiment of a Large Measurement Range Flexible Joints 6-UPUR Six-Axis Force Sensor
Previous Article in Journal
Vertical Corner Feature Based Precise Vehicle Localization Using 3D LIDAR in Urban Area
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(8), 1274; doi:10.3390/s16081274

Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element

1
TÜBİTAK, The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, 41470 Kocaeli, Turkey
2
Department of Molecular Biology and Genetics, Gebze Technical University, 41400 Kocaeli, Turkey
3
Department of Physics, Gebze Technical University, 41400 Kocaeli, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 15 May 2016 / Revised: 17 July 2016 / Accepted: 29 July 2016 / Published: 11 August 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [2212 KB, uploaded 11 August 2016]   |  

Abstract

This study introduces the use of an IgA isotype aflatoxin (AF) specific monoclonal antibody for the development of a highly sensitive Quartz Crystal Microbalance (QCM) immunobiosensor for the detection of AF in inhibitory immunoassay format. The higher molecular weight of IgA antibodies proved an advantage over commonly used IgG antibodies in label free immunobiosensor measurements. IgA and IgG antibodies with similar affinity for AF were used in the comparative studies. Sensor surface was prepared by covalent immobilization of AFB1, using self assembled monolayer (SAM) formed on gold coated Quartz Crystal, with 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy succinimide (EDC/NHS) method using a diamine linker. Nonspecific binding to the surface was decreased by minimizing the duration of EDC/NHS activation. Sensor surface was chemically blocked after AF immobilization without any need for protein blocking. This protein free sensor chip endured harsh solutions with strong ionic detergent at high pH, which is required for the regeneration of the high affinity antibody-antigen interaction. According to the obtained results, the detection range with IgA antibodies was higher than IgG antibodies in QCM immunosensor developed for AFB1. View Full-Text
Keywords: QCM immunosensor; IgA monoclonal antibody; aflatoxin immobilization; chemical blocking QCM immunosensor; IgA monoclonal antibody; aflatoxin immobilization; chemical blocking
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ertekin, Ö.; Öztürk, S.; Öztürk, Z.Z. Label Free QCM Immunobiosensor for AFB1 Detection Using Monoclonal IgA Antibody as Recognition Element. Sensors 2016, 16, 1274.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top