Next Article in Journal
Theoretical and Experimental Study on Wide Range Optical Fiber Turbine Flow Sensor
Previous Article in Journal
Correcting Spatial Variance of RCM for GEO SAR Imaging Based on Time-Frequency Scaling
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(7), 1094; doi:10.3390/s16071094

Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis

1,5,†
,
1,2,†,* , 1,2,3,4,* , 1,2,3,* and 1,2,5
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
4
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
5
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 1 May 2016 / Revised: 8 June 2016 / Accepted: 6 July 2016 / Published: 14 July 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [961 KB, uploaded 14 July 2016]   |  

Abstract

Rapid and quantitative sensing of aflatoxin B1 with high sensitivity and specificity has drawn increased attention of studies investigating soybean sauce. A sensitive and rapid quantitative immunochromatographic sensing method was developed for the detection of aflatoxin B1 based on time-resolved fluorescence. It combines the advantages of time-resolved fluorescent sensing and immunochromatography. The dynamic range of a competitive and portable immunoassay was 0.3–10.0 µg·kg−1, with a limit of detection (LOD) of 0.1 µg·kg−1 and recoveries of 87.2%–114.3%, within 10 min. The results showed good correlation (R2 > 0.99) between time-resolved fluorescent immunochromatographic strip test and high performance liquid chromatography (HPLC). Soybean sauce samples analyzed using time-resolved fluorescent immunochromatographic strip test revealed that 64.2% of samples contained aflatoxin B1 at levels ranging from 0.31 to 12.5 µg·kg−1. The strip test is a rapid, sensitive, quantitative, and cost-effective on-site screening technique in food safety analysis. View Full-Text
Keywords: time-resolved fluorescent immunochromatographic strip; aflatoxin B1; soybean sauce; food safety time-resolved fluorescent immunochromatographic strip; aflatoxin B1; soybean sauce; food safety
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, D.; Zhang, Z.; Li, P.; Zhang, Q.; Zhang, W. Time-Resolved Fluorescent Immunochromatography of Aflatoxin B1 in Soybean Sauce: A Rapid and Sensitive Quantitative Analysis. Sensors 2016, 16, 1094.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top