Next Article in Journal
Sorption and Diffusion of Water Vapor and Carbon Dioxide in Sulfonated Polyaniline as Chemical Sensing Materials
Previous Article in Journal
Numerical Simulation of Output Response of PVDF Sensor Attached on a Cantilever Beam Subjected to Impact Loading
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(5), 602; doi:10.3390/s16050602

Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones

Institute of Genetics, Technische Universität Dresden, 01062 Dresden, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: S. Scott Saavedra
Received: 10 March 2016 / Revised: 31 March 2016 / Accepted: 20 April 2016 / Published: 27 April 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [2567 KB, uploaded 27 April 2016]   |  

Abstract

Detection and quantification of small peptides, such as yeast pheromones, are often challenging. We developed a highly sensitive and robust affinity-assay for the quantification of the α-factor pheromone of Saccharomyces cerevisiae based on recombinant hydrophobins. These small, amphipathic proteins self-assemble into highly stable monolayers at hydrophilic-hydrophobic interfaces. Upon functionalization of solid supports with a combination of hydrophobins either lacking or exposing the α-factor, pheromone-specific antibodies were bound to the surface. Increasing concentrations of the pheromone competitively detached the antibodies, thus allowing for quantification of the pheromone. By adjusting the percentage of pheromone-exposing hydrophobins, the sensitivity of the assay could be precisely predefined. The assay proved to be highly robust against changes in sample matrix composition. Due to the high stability of hydrophobin layers, the functionalized surfaces could be repeatedly used without affecting the sensitivity. Furthermore, by using an inverse setup, the sensitivity was increased by three orders of magnitude, yielding a novel kind of biosensor for the yeast pheromone with the lowest limit of detection reported so far. This assay was applied to study the pheromone secretion of diverse yeast strains including a whole-cell biosensor strain of Schizosaccharomyces pombe modulating α-factor secretion in response to an environmental signal. View Full-Text
Keywords: analyte detection; yeast pheromone; hydrophobin; surface functionalization; whole-cell biosensor; biosensor analyte detection; yeast pheromone; hydrophobin; surface functionalization; whole-cell biosensor; biosensor
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hennig, S.; Rödel, G.; Ostermann, K. Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones. Sensors 2016, 16, 602.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top