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Sensors 2016, 16(4), 535; doi:10.3390/s16040535

An Easily Fabricated Electrochemical Sensor Based on a Graphene-Modified Glassy Carbon Electrode for Determination of Octopamine and Tyramine

1
Research Center for Bioengineering and Sensing Technology, University of Science and Technology, Beijing 100083, China
2
Department of Zoology, College of Science, P. O. Box 2455, King Saud University, Riyadh 11451, Saudi Arabia
3
Department of Zoology, Government College University, Fsisalabad 38000, Pakistan
*
Authors to whom correspondence should be addressed.
Academic Editor: Gregory Schneider
Received: 4 March 2016 / Revised: 5 April 2016 / Accepted: 11 April 2016 / Published: 13 April 2016
(This article belongs to the Special Issue Graphene and 2D Material Bionanosensors: Chemistry Matters)
View Full-Text   |   Download PDF [2486 KB, uploaded 13 April 2016]   |  

Abstract

A simple electrochemical sensor has been developed for highly sensitive detection of octopamine and tyramine by electrodepositing reduced graphene oxide (ERGO) nanosheets onto the surface of a glassy carbon electrode (GCE). The electrocatalytic oxidation of octopamine and tyramine is individually investigated at the surface of the ERGO modified glassy carbon electrode (ERGO/GCE) by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Several essential factors including the deposition cycle of reduced graphene oxide nanosheets and the pH of the running buffer were investigated in order to determine the optimum conditions. Furthermore, the sensor was applied to the quantification of octopamine and tyramine by DPV in the concentration ranges from 0.5 to 40 μM and 0.1 to 25 μM, respectively. In addition, the limits of detection of octopamine and tyramine were calculated to be 0.1 μM and 0.03 μM (S/N = 3), respectively. The sensor showed good reproducibility, selectivity and stability. Finally, the sensor successfully detected octopamine and tyramine in commercially available beer with satisfactory recovery ranges which were 98.5%–104.7% and 102.2%–103.1%, respectively. These results indicate the ERGO/GCE based sensor is suitable for the detection of octopamine and tyramine. View Full-Text
Keywords: octopamine; tyramine; graphene; electrochemical sensor octopamine; tyramine; graphene; electrochemical sensor
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MDPI and ACS Style

Zhang, Y.; Zhang, M.; Wei, Q.; Gao, Y.; Guo, L.; Al-Ghanim, K.A.; Mahboob, S.; Zhang, X. An Easily Fabricated Electrochemical Sensor Based on a Graphene-Modified Glassy Carbon Electrode for Determination of Octopamine and Tyramine. Sensors 2016, 16, 535.

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