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Sensors 2016, 16(3), 376; doi:10.3390/s16030376

Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains

1
Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea
2
Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea
3
Korea Mycobacterium Resource Center (KMRC), The Korean Institute of Tuberculosis, Cheongju-si 28158, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 28 January 2016 / Revised: 8 March 2016 / Accepted: 11 March 2016 / Published: 15 March 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1288 KB, uploaded 15 March 2016]   |  

Abstract

In 2013 alone, the death rate among the 9.0 million people infected with Mycobacterium tuberculosis (TB) worldwide was around 14%, which is unacceptably high. An empiric treatment of patients infected with TB or drug-resistant Mycobacterium tuberculosis (MDR-TB) strain can also result in the spread of MDR-TB. The diagnostic tools which are rapid, reliable, and have simple experimental protocols can significantly help in decreasing the prevalence rate of MDR-TB strain. We report the evaluation of the 9G technology based 9G DNAChips that allow accurate detection and discrimination of TB and MDR-TB-RIF. One hundred and thirteen known cultured samples were used to evaluate the ability of 9G DNAChip in the detection and discrimination of TB and MDR-TB-RIF strains. Hybridization of immobilized probes with the PCR products of TB and MDR-TB-RIF strains allow their detection and discrimination. The accuracy of 9G DNAChip was determined by comparing its results with sequencing analysis and drug susceptibility testing. Sequencing analysis showed 100% agreement with the results of 9G DNAChip. The 9G DNAChip showed very high sensitivity (95.4%) and specificity (100%). View Full-Text
Keywords: tuberculosis; drug-resistant tuberculosis; diagnosis; accuracy; sensitivity; specificity tuberculosis; drug-resistant tuberculosis; diagnosis; accuracy; sensitivity; specificity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Song, K.-S.; Nimse, S.B.; Kim, H.J.; Yang, J.; Kim, T. Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains. Sensors 2016, 16, 376.

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