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Sensors 2016, 16(3), 272; doi:10.3390/s16030272

Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis

1
Institute of Biochemistry and Biology, Potsdam University, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam, Germany
2
Institute of Biology, Structural Biology/Biochemistry, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin, Germany
3
Fraunhofer Institute for Cell Therapy and Immunology IZI-BB, Am Mühlenberg 13, 14476 Potsdam, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 20 January 2016 / Revised: 12 February 2016 / Accepted: 18 February 2016 / Published: 23 February 2016
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Abstract

For the first time a molecularly imprinted polymer (MIP) with direct electron transfer (DET) and bioelectrocatalytic activity of the target protein is presented. Thin films of MIPs for the recognition of a hexameric tyrosine-coordinated heme protein (HTHP) have been prepared by electropolymerization of scopoletin after oriented assembly of HTHP on a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold electrodes. Cavities which should resemble the shape and size of HTHP were formed by template removal. Rebinding of the target protein sums up the recognition by non-covalent interactions between the protein and the MIP with the electrostatic attraction of the protein by the SAM. HTHP bound to the MIP exhibits quasi-reversible DET which is reflected by a pair of well pronounced redox peaks in the cyclic voltammograms (CVs) with a formal potential of −184.4 ± 13.7 mV vs. Ag/AgCl (1 M KCl) at pH 8.0 and it was able to catalyze the cathodic reduction of peroxide. At saturation the MIP films show a 12-fold higher electroactive surface concentration of HTHP than the non-imprinted polymer (NIP). View Full-Text
Keywords: molecularly imprinted polymers; self-assembled monolayer; direct electron transfer; hydrogen peroxide; bioelectrocatalysis molecularly imprinted polymers; self-assembled monolayer; direct electron transfer; hydrogen peroxide; bioelectrocatalysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Peng, L.; Yarman, A.; Jetzschmann, K.J.; Jeoung, J.-H.; Schad, D.; Dobbek, H.; Wollenberger, U.; Scheller, F.W. Molecularly Imprinted Electropolymer for a Hexameric Heme Protein with Direct Electron Transfer and Peroxide Electrocatalysis. Sensors 2016, 16, 272.

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