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Sensors 2016, 16(12), 2179; doi:10.3390/s16122179

Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media

1
Urban Robotics Laboratory, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea
2
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea
3
School of Electrical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Amine Miled and Jesse Greener
Received: 7 November 2016 / Revised: 4 December 2016 / Accepted: 14 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Microfluidics-Based Microsystem Integration Research)
View Full-Text   |   Download PDF [2551 KB, uploaded 20 December 2016]   |  

Abstract

The detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern marker. The quantification of bacterial growth is possible as the bacterial growth in the culturing vessel blurs the marker image, which is placed on the back of the vessel, and the blurring results in a decrease in the high-frequency spectrum region of the marker image. The experiment results show that the FFT (fast Fourier transform)-based growth detection method is robust to the variations in the type of bacterial carrier and vessels ranging from the culture tubes to the microfluidic devices. Moreover, the automated incubator and image acquisition system are developed to be used as a comprehensive in situ detection system. We expect that this result can be applied in the automation of biological experiments, such as the Antibiotics Susceptibility Test or toxicity measurement. Furthermore, the simple framework of the proposed growth measurement method may be further utilized as an effective and convenient method for building point-of-care devices for developing countries. View Full-Text
Keywords: bacterial growth; vision marker; fast Fourier transformation; microfluidics bacterial growth; vision marker; fast Fourier transformation; microfluidics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, K.; Choi, D.; Lim, H.; Kim, H.; Jeon, J.S. Vision Marker-Based In Situ Examination of Bacterial Growth in Liquid Culture Media. Sensors 2016, 16, 2179.

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