Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells
AbstractManipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6′-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B. The zeta potential of the nanosensor switches between negative and positive by photo-isomerization of SP with ultraviolet irradiation. The positively-charged nanosensor easily adheres to a negatively-charged glass nanoprobe, is transported to a target cell, and then adheres to the negatively-charged cell membrane. The nanosensor is then injected into the cytoplasm by heating with a near-infrared (NIR) laser. As a demonstration, a single 750 nm nanosensor was picked-up using a glass nanoprobe with optical control of the zeta potential. Then, the nanosensor was transported and immobilized onto a target cell membrane. Finally, it was injected into the cytoplasm using a NIR laser. The success rates of pick-up and cell immobilization of the nanosensor were 75% and 64%, respectively. Cell injection and cell survival rates were 80% and 100%, respectively. View Full-Text
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Hashim, H.; Maruyama, H.; Masuda, T.; Arai, F. Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells. Sensors 2016, 16, 2041.
Hashim H, Maruyama H, Masuda T, Arai F. Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells. Sensors. 2016; 16(12):2041.Chicago/Turabian Style
Hashim, Hairulazwan; Maruyama, Hisataka; Masuda, Taisuke; Arai, Fumihito. 2016. "Manipulation and Immobilization of a Single Fluorescence Nanosensor for Selective Injection into Cells." Sensors 16, no. 12: 2041.
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