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Sensors 2016, 16(11), 1901; doi:10.3390/s16111901

Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater

1
SensorLab, Department of Chemistry, University of Western Cape, Robert Sobukwe Road, Bellville, Cape Town 7535, South Africa
2
Department of Biological and Chemical Sciences, The University of the West Indies, Cave Hill, St. Michael BB11000, Barbados
*
Author to whom correspondence should be addressed.
Academic Editor: Beate Strehlitz
Received: 7 August 2016 / Revised: 19 October 2016 / Accepted: 20 October 2016 / Published: 11 November 2016
(This article belongs to the Special Issue Aptasensors 2016)
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Abstract

A sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD–Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, l-leucine; R, l-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD–Co(II)|AgNPs aptatoxisensor was fabricated with 5’ thiolated aptamer through self-assembly on the modified surface of the glassy carbon electrode (GCE) and the electronic response was measured using cyclic voltammetry (CV). Specific binding of MC-LR with the aptamer on GCE|SDD–Co(II)|AgNPs aptatoxisensor caused the formation of a complex that resulted in steric hindrance and electrostatic repulsion culminating in variation of the corresponding peak current of the electrochemical probe. The aptatoxisensor showed a linear response for MC-LR between 0.1 and 1.1 µg·L−1 and the calculated limit of detection (LOD) was 0.04 µg·L−1. In the detection of MC-LR in water samples, the aptatoxisensor proved to be highly sensitive and stable, performed well in the presence of interfering analog and was comparable to the conventional analytical techniques. The results demonstrate that the constructed MC-LR aptatoxisensor is a suitable device for routine quantification of MC-LR in freshwater and environmental samples. View Full-Text
Keywords: aptamer; aptatoxisensor; cyanotoxins; electrochemical biosensor; metallodendrimer; microcystin-LR; nanosensor aptamer; aptatoxisensor; cyanotoxins; electrochemical biosensor; metallodendrimer; microcystin-LR; nanosensor
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MDPI and ACS Style

Bilibana, M.P.; Williams, A.R.; Rassie, C.; Sunday, C.E.; Makelane, H.; Wilson, L.; Ntshongontshi, N.; Jijana, A.N.; Masikini, M.; Baker, P.G.L.; Iwuoha, E.I. Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater. Sensors 2016, 16, 1901.

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