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Sensors 2016, 16(11), 1856; doi:10.3390/s16111856

The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7

1
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 45002, China
2
State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 45002, China
3
College of life sciences, Henan Agricultural University, Zhengzhou 45002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Meyya Meyyappan
Received: 12 September 2016 / Revised: 18 October 2016 / Accepted: 1 November 2016 / Published: 4 November 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1096 KB, uploaded 7 November 2016]   |  

Abstract

The purpose of this study was to develop a portable surface plasmon resonance (SPR) bioanalyzer for the sensitive detection of Escherichia coli O157:H7 in comparison with an enzyme-linked immunosorbent assay (ELISA). The experimental setup mainly consisted of an integrated biosensor and a homemade microfluidic cell with a three-way solenoid valve. In order to detect Escherichia coli O157:H7 using the SPR immunoassay, 3-mercaptopropionic acid (3-MPA) was chemisorbed onto a gold surface via covalent bond for the immobilization of biological species. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were used as crosslinker reagents to enable the reaction between 3-MPA and Escherichia coli O157:H7 antibodies by covalent –CO–NH– amide bonding. The experimental results were obtained from the Escherichia coli O157:H7 positive samples prepared by 10-, 20-, 40-, 80-, and 160-fold dilution respectively, which show that a good linear relationship with the correlation coefficient R of 0.982 existed between the response units from the portable SPR bioanalyzer and the concentration of Escherichia coli O157:H7 positive samples. Moreover, the theoretical detection limit of 1.87 × 103 cfu/mL was calculated from the positive control samples. Compared with the Escherichia coli O157:H7 ELISA kit, the sensitivity of this portable SPR bioanalyzer is four orders of magnitude higher than the ELISA kit. The results demonstrate that the portable SPR bioanalyzer could provide an alternative method for the quantitative and sensitive determination of Escherichia coli O157:H7 in field. View Full-Text
Keywords: Escherichia coli O157:H7; portable surface plasmon resonance (SPR) bioanalyzer; biomolecular recognition membrane; ELISA kit; response unit Escherichia coli O157:H7; portable surface plasmon resonance (SPR) bioanalyzer; biomolecular recognition membrane; ELISA kit; response unit
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, S.; Xie, J.; Jiang, M.; Chang, K.; Chen, R.; Ma, L.; Zhu, J.; Guo, Q.; Sun, H.; Hu, J. The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors 2016, 16, 1856.

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