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Sensors 2016, 16(11), 1808; doi:10.3390/s16111808

Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16

1
Institute of Scientific Instruments of the CAS, Brno 61264, Czech Republic
2
Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Brno 61200, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Sebastian Wachsmann-Hogiu and Zachary J. Smith
Received: 28 July 2016 / Revised: 14 October 2016 / Accepted: 18 October 2016 / Published: 28 October 2016
(This article belongs to the Special Issue Applications of Raman Spectroscopy in Biosensors)
View Full-Text   |   Download PDF [949 KB, uploaded 28 October 2016]   |  

Abstract

We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800–1800 cm−1) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm−1) and for the selection of the two internal standards (DNA at 786 cm−1 and Amide I at 1662 cm−1). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts—determined by gas chromatography—from 12% to 90% (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure—from bacteria sampling, centrifugation, and sample preparation to Raman analysis—can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h. View Full-Text
Keywords: Raman spectroscopy; Cupriavidus necator H16; polyhydroxyalkanoates Raman spectroscopy; Cupriavidus necator H16; polyhydroxyalkanoates
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MDPI and ACS Style

Samek, O.; Obruča, S.; Šiler, M.; Sedláček, P.; Benešová, P.; Kučera, D.; Márova, I.; Ježek, J.; Bernatová, S.; Zemánek, P. Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16. Sensors 2016, 16, 1808.

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