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Sensors 2016, 16(10), 1761; doi:10.3390/s16101761

Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application

1
Department of Applied Physics, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan
2
Department of Food Science and Biotechnology, Kagoshima University, Korimoto, Kagoshima 1-21-24, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 15 July 2016 / Revised: 12 September 2016 / Accepted: 23 September 2016 / Published: 21 October 2016
(This article belongs to the Special Issue Point-of-Care Biosensors)
View Full-Text   |   Download PDF [2723 KB, uploaded 21 October 2016]   |  

Abstract

In this review we present electrochemical biosensor developments, focusing on screen-printed electrodes (SPEs) and their applications. In particular, we discuss how SPEs enable simple integration, and the portability needed for on-field applications. First, we briefly discuss the general concept of biosensors and quickly move on to electrochemical biosensors. Drawing from research undertaken in this area, we cover the development of electrochemical DNA biosensors in great detail. Through specific examples, we describe the fabrication and surface modification of printed electrodes for sensitive and selective detection of targeted DNA sequences, as well as integration with reverse transcription-polymerase chain reaction (RT-PCR). For a more rounded approach, we also touch on electrochemical immunosensors and enzyme-based biosensors. Last, we present some electrochemical devices specifically developed for use with SPEs, including USB-powered compact mini potentiostat. The coupling demonstrates the practical use of printable electrode technologies for application at point-of-use. Although tremendous advances have indeed been made in this area, a few challenges remain. One of the main challenges is application of these technologies for on-field analysis, which involves complicated sample matrices. View Full-Text
Keywords: biosensors; electrochemical; point-of-use; screen-printed electrodes (SPEs); disposable electrochemical printed (DEP) chips biosensors; electrochemical; point-of-use; screen-printed electrodes (SPEs); disposable electrochemical printed (DEP) chips
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yamanaka, K.; Vestergaard, M.C.; Tamiya, E. Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application. Sensors 2016, 16, 1761.

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