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Sensors 2015, 15(8), 19225-19236; doi:10.3390/s150819225

Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors

1
Nanjing Medical University, Nanjing 210029, China
2
Wuxi People's Hospital, Wuxi 214023, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 1 June 2015 / Revised: 22 July 2015 / Accepted: 27 July 2015 / Published: 5 August 2015
(This article belongs to the Section Biosensors)
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Abstract

Primary hepatic carcinoma (PHC) is one of the most common malignancies worldwide, resulting in death within six to 20 months. The survival rate can be improved by effective treatments when diagnosed at an early stage. The α-fetoprotein (AFP) and carcinoembryonic antigen (CEA) have been identified as markers that are expressed at higher levels in PHC patients. In this study, we employed silicon nanowire field-effect transistors (SiNW-FETs) with polydimethylsiloxane (PDMS) microfluidic channels to simultaneously detect AFP and CEA in desalted human serum. Dual-channel PDMS was first utilized for the selective modification of AFP and CEA antibodies on SiNWs, while single-channel PDMS offers faster and more sensitive detection of AFP and CEA in serum. During the SiNW modification process, 0.1% BSA was utilized to minimize nonspecific protein binding from serum. The linear dynamic ranges for the AFP and CEA detection were measured to be 500 fg/mL to 50 ng/mL and 50 fg/mL to 10 ng/mL, respectively. Our work demonstrates the promising potential of fabricated SiNW-FETs as a direct detection kit for multiple tumor markers in serum; therefore, it provides a chance for early stage diagnose and, hence, more effective treatments for PHC patients. View Full-Text
Keywords: primary hepatic carcinoma; silicon nanowire field-effect transistor; α-fetoprotein; carcinoembryonic antigen; polydimethylsiloxane microfluidic channel primary hepatic carcinoma; silicon nanowire field-effect transistor; α-fetoprotein; carcinoembryonic antigen; polydimethylsiloxane microfluidic channel
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhu, K.; Zhang, Y.; Li, Z.; Zhou, F.; Feng, K.; Dou, H.; Wang, T. Simultaneous Detection of α-Fetoprotein and Carcinoembryonic Antigen Based on Si Nanowire Field-Effect Transistors. Sensors 2015, 15, 19225-19236.

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