Next Article in Journal
Spectral and Image Integrated Analysis of Hyperspectral Data for Waxy Corn Seed Variety Classification
Next Article in Special Issue
PEG Functionalization of Whispering Gallery Mode Optical Microresonator Biosensors to Minimize Non-Specific Adsorption during Targeted, Label-Free Sensing
Previous Article in Journal
Improving Localization Accuracy: Successive Measurements Error Modeling
Previous Article in Special Issue
Diamond Nanowires: A Novel Platform for Electrochemistry and Matrix-Free Mass Spectrometry
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(7), 15562-15577; doi:10.3390/s150715562

A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences

1
Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego, s/n, Campus da UFPE, 50670-901 Recife, PE, Brazil
2
Universidade Federal de Alagoas (UFAL), Campus Arapiraca, Av. Manoel Severino Barbosa, s/n, Bom Sucesso, 57.309-005 Arapiraca, AL, Brazil
3
Departamento de Virologia e Terapia Experimental (LAVITE), Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (Fiocruz)—Pernambuco, Av. Professor Moraes Rego, s/n, Campus da UFPE, 50.670-420 Recife, PE, Brazil
4
Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Professor Moraes Rego, s/n, Campus da UFPE, CEP: 50670-901 Recife, PE, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen Holler
Received: 1 March 2015 / Revised: 13 June 2015 / Accepted: 23 June 2015 / Published: 1 July 2015
(This article belongs to the Special Issue Label-Free Sensing)
View Full-Text   |   Download PDF [1194 KB, uploaded 1 July 2015]   |  

Abstract

Dengue fever is the most prevalent vector-borne disease in the world, with nearly 100 million people infected every year. Early diagnosis and identification of the pathogen are crucial steps for the treatment and for prevention of the disease, mainly in areas where the co-circulation of different serotypes is common, increasing the outcome of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Due to the lack of fast and inexpensive methods available for the identification of dengue serotypes, herein we report the development of an electrochemical DNA biosensor for the detection of sequences of dengue virus serotype 3 (DENV-3). DENV-3 probe was designed using bioinformatics software and differential pulse voltammetry (DPV) was used for electrochemical analysis. The results showed that a 22-m sequence was the best DNA probe for the identification of DENV-3. The optimum concentration of the DNA probe immobilized onto the electrode surface is 500 nM and a low detection limit of the system (3.09 nM). Moreover, this system allows selective detection of DENV-3 sequences in buffer and human serum solutions. Therefore, the application of DNA biosensors for diagnostics at the molecular level may contribute to future advances in the implementation of specific, effective and rapid detection methods for the diagnosis dengue viruses. View Full-Text
Keywords: dengue fever; DNA biosensors; differential pulse voltammetry; electrochemical detection; guanine oxidation dengue fever; DNA biosensors; differential pulse voltammetry; electrochemical detection; guanine oxidation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Oliveira, N.; Souza, E.; Ferreira, D.; Zanforlin, D.; Bezerra, W.; Borba, M.A.; Arruda, M.; Lopes, K.; Nascimento, G.; Martins, D.; Cordeiro, M.; Lima-Filho, J. A Sensitive and Selective Label-Free Electrochemical DNA Biosensor for the Detection of Specific Dengue Virus Serotype 3 Sequences. Sensors 2015, 15, 15562-15577.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top