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Sensors 2015, 15(5), 9666-9680; doi:10.3390/s150509666

Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection

1
CNR—Institute of Applied Physics "Nello Carrara", Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy
2
Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Piazza del Viminale 1, 00184 Rome, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Ashutosh Tiwari
Received: 4 February 2015 / Revised: 2 April 2015 / Accepted: 20 April 2015 / Published: 24 April 2015
(This article belongs to the Special Issue Smart Materials for Switchable Sensors)
View Full-Text   |   Download PDF [705 KB, uploaded 29 April 2015]   |  

Abstract

Optical fiber sensors, thanks to their compactness, fast response and real-time measurements, have a large impact in the fields of life science research, drug discovery and medical diagnostics. In recent years, advances in nanotechnology have resulted in the development of nanotools, capable of entering the single cell, resulting in new nanobiosensors useful for the detection of biomolecules inside living cells. In this paper, we provide an application of a nanotip coupled with molecular beacons (MBs) for the detection of DNA. The MBs were characterized by hybridization studies with a complementary target to prove their functionality both free in solution and immobilized onto a solid support. The solid support chosen as substrate for the immobilization of the MBs was a 30 nm tapered tip of an optical fiber, fabricated by chemical etching. With this set-up promising results were obtained and a limit of detection (LOD) of 0.57 nM was reached, opening up the possibility of using the proposed nanotip to detect mRNAs inside the cytoplasm of living cells. View Full-Text
Keywords: molecular beacon; optical fiber nanotip; nanosensor; optical biosensors; fluorescence; mRNA; DNA; survivin molecular beacon; optical fiber nanotip; nanosensor; optical biosensors; fluorescence; mRNA; DNA; survivin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Giannetti, A.; Barucci, A.; Cosi, F.; Pelli, S.; Tombelli, S.; Trono, C.; Baldini, F. Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection. Sensors 2015, 15, 9666-9680.

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