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Sensors 2015, 15(3), 5281-5292; doi:10.3390/s150305281

A Highly Sensitive ELISA and Immunochromatographic Strip for the Detection of Salmonella typhimurium in Milk Samples

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
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Academic Editor: Alexander Star
Received: 21 January 2015 / Revised: 16 February 2015 / Accepted: 18 February 2015 / Published: 4 March 2015
(This article belongs to the Section Biosensors)
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Abstract

Murine monoclonal antibodies to target Salmonella typhimurium flagellin and lipopolysaccharide (LPS) were prepared and characterized. For the immunological detection of S. typhimurium, different pairs of monoclonal antibodies (MAbs) were tested in a sandwich enzyme linked immunosorbent assay (ELISA) format. After comparison, a sandwich ELISA and immunochromatographic strip based on LPS MAbs was established to detect S. typhimurium. The determination limits of the immunochromatographic strip in phosphate-buffered saline (PBS) containing 0.1% Tween 20 (PBST) and pure milk sample were found to be 1.25 × 105 colony-forming units (cfu)/mL and 1.25 × 106 cfu/mL S. typhimurium, respectively. Results can be obtained with the naked eye in 10 min. Cross-reactivity was observed with Salmonella paratyphi B, but not S. paratyphi A or Salmonella enteritidis. The LPS MAbs based immunochromatographic strip is rapid and convenient to detect S. typhimurium in milk samples. View Full-Text
Keywords: Salmonella typhimurium; flagellin; LPS; monoclonal antibody; sandwich ELISA; immunochromatographic strip; detection Salmonella typhimurium; flagellin; LPS; monoclonal antibody; sandwich ELISA; immunochromatographic strip; detection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, W.; Liu, L.; Song, S.; Tang, L.; Kuang, H.; Xu, C. A Highly Sensitive ELISA and Immunochromatographic Strip for the Detection of Salmonella typhimurium in Milk Samples. Sensors 2015, 15, 5281-5292.

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