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Sensors 2015, 15(2), 2798-2811; doi:10.3390/s150202798

Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate

1
Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Avenida Normalistas 800, Colinas de la Normal. C.P. 44270, Guadalajara Jalisco, Mexico
2
Departament d'Enginyeria Química, Escola d'Enginyeria, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
3
Departament de Química, Facultat de Ciències, Edifici C-Nord, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
4
Grupo de Biodinámica y Sistemas Alineales, División de Matemáticas Aplicadas, Instituto Potosinode Investigación Científicay Tecnológica. A.C. Camino a la Presa San José 2055, Lomas 4 Sección, C.P. 78216, San Luis Potosí S.L.P., Mexico
*
Author to whom correspondence should be addressed.
Received: 1 November 2014 / Revised: 24 December 2014 / Accepted: 13 January 2015 / Published: 27 January 2015
(This article belongs to the Special Issue Sensors for Bioprocess Monitoring and Control)
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Abstract

Lipases and esterases are biocatalysts used at the laboratory and industrial level. To obtain the maximum yield in a bioprocess, it is important to measure key variables, such as enzymatic activity. The conventional method for monitoring hydrolytic activity is to take out a sample from the bioreactor to be analyzed off-line at the laboratory. The disadvantage of this approach is the long time required to recover the information from the process, hindering the possibility to develop control systems. New strategies to monitor lipase/esterase activity are necessary. In this context and in the first approach, we proposed a lab-made sequential injection analysis system to analyze off-line samples from shake flasks. Lipase/esterase activity was determined using p-nitrophenyl butyrate as the substrate. The sequential injection analysis allowed us to measure the hydrolytic activity from a sample without dilution in a linear range from 0.05–1.60 U/mL, with the capability to reach sample dilutions up to 1000 times, a sampling frequency of five samples/h, with a kinetic reaction of 5 min and a relative standard deviation of 8.75%. The results are promising to monitor lipase/esterase activity in real time, in which optimization and control strategies can be designed. View Full-Text
Keywords: monitoring; lipase/esterase activity; sequential injection analysis; stopped flow; p-nitrophenyl esters monitoring; lipase/esterase activity; sequential injection analysis; stopped flow; p-nitrophenyl esters
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Pliego, J.; Mateos, J.C.; Rodriguez, J.; Valero, F.; Baeza, M.; Femat, R.; Camacho, R.; Sandoval, G.; Herrera-López, E.J. Monitoring Lipase/Esterase Activity by Stopped Flow in a Sequential Injection Analysis System Using p-Nitrophenyl Butyrate. Sensors 2015, 15, 2798-2811.

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