Sensors 2014, 14(7), 13298-13307; doi:10.3390/s140713298
Article

Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles

1,2,3,* email, 3email, 4email, 5email, 6email and 1email
Received: 7 April 2014; in revised form: 17 June 2014 / Accepted: 17 July 2014 / Published: 23 July 2014
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 µs. These results are greater than that found without any nanoparticles (maximum response of 10 µs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.
Keywords: gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement
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MDPI and ACS Style

Nouira, W.; Maaref, A.; Elaissari, H.; Vocanson, F.; Siadat, M.; Jaffrezic-Renault, N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors 2014, 14, 13298-13307.

AMA Style

Nouira W, Maaref A, Elaissari H, Vocanson F, Siadat M, Jaffrezic-Renault N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors. 2014; 14(7):13298-13307.

Chicago/Turabian Style

Nouira, Wided; Maaref, Abderrazak; Elaissari, Hamid; Vocanson, Francis; Siadat, Maryam; Jaffrezic-Renault, Nicole. 2014. "Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles." Sensors 14, no. 7: 13298-13307.

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