Abstract: Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 µs. These results are greater than that found without any nanoparticles (maximum response of 10 µs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.
Keywords: gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement
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Nouira, W.; Maaref, A.; Elaissari, H.; Vocanson, F.; Siadat, M.; Jaffrezic-Renault, N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors 2014, 14, 13298-13307.
Nouira W, Maaref A, Elaissari H, Vocanson F, Siadat M, Jaffrezic-Renault N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors. 2014; 14(7):13298-13307.
Nouira, Wided; Maaref, Abderrazak; Elaissari, Hamid; Vocanson, Francis; Siadat, Maryam; Jaffrezic-Renault, Nicole. 2014. "Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles." Sensors 14, no. 7: 13298-13307.