Next Article in Journal
Temperature Measurement in PV Facilities on a Per-Panel Scale
Previous Article in Journal
Strategy for Making a Superior Quenchbody to Proteins: Effect of the Fluorophore Position
Sensors 2014, 14(7), 13298-13307; doi:10.3390/s140713298
Article

Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles

1,2,3,* , 3
, 4
, 5
, 6
 and 1
1 Institute of Analytical Sciences, University of Lyon, La Doua Street, 5, 69622 Villeurbanne, France 2 Department of Physics, College of Science, Qassim University, Buraidah 51452, Saudi Arabia 3 Laboratory of Interfaces and Advanced Materials, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia 4 University of Lyon, CNRS, UMR 5007, LAGEP-CPE, 43 Bd. 11 Novembre 1918, Villeurbanne, France 5 Hubert Curien Laboratory, University of Lyon, F-42023 Saint-Etienne, France 6 LASC, ISEA, University of Metz, 7 Marconi Street, 57070 Metz, France
* Author to whom correspondence should be addressed.
Received: 7 April 2014 / Revised: 17 June 2014 / Accepted: 17 July 2014 / Published: 23 July 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [308 KB, 24 July 2014; original version 23 July 2014]   |   Browse Figures

Abstract

Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 µs. These results are greater than that found without any nanoparticles (maximum response of 10 µs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.
Keywords: gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Nouira, W.; Maaref, A.; Elaissari, H.; Vocanson, F.; Siadat, M.; Jaffrezic-Renault, N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors 2014, 14, 13298-13307.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert