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Sensors 2014, 14(7), 13298-13307; doi:10.3390/s140713298
Article

Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles

1,2,3,* , 3
,
4
,
5
,
6
 and
1
1 Institute of Analytical Sciences, University of Lyon, La Doua Street, 5, 69622 Villeurbanne, France 2 Department of Physics, College of Science, Qassim University, Buraidah 51452, Saudi Arabia 3 Laboratory of Interfaces and Advanced Materials, University of Monastir, Avenue of Environment, 5019 Monastir, Tunisia 4 University of Lyon, CNRS, UMR 5007, LAGEP-CPE, 43 Bd. 11 Novembre 1918, Villeurbanne, France 5 Hubert Curien Laboratory, University of Lyon, F-42023 Saint-Etienne, France 6 LASC, ISEA, University of Metz, 7 Marconi Street, 57070 Metz, France
* Author to whom correspondence should be addressed.
Received: 7 April 2014 / Revised: 17 June 2014 / Accepted: 17 July 2014 / Published: 23 July 2014
(This article belongs to the Section Biosensors)
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Abstract

Proteinases are involved in a multitude of important physiological processes, such as protein metabolism. For this reason, a conductometric enzyme biosensor based on proteinase K was developed using two types of nanoparticles (gold and magnetic). The enzyme was directly adsorbed on negatively charged nanoparticles and then deposited and cross-linked on a planar interdigitated electrode (IDE). The biosensor was characterized with bovine serum albumin (BSA) as a standard protein. Higher sensitivity was obtained using gold nanoparticles. The linear range for BSA determination was then from 0.5 to 10 mg/L with a maximum response of 154 µs. These results are greater than that found without any nanoparticles (maximum response of 10 µs). The limit of detection (LOD) was 0.3 mg/L. An inter-sensor reproducibility of 3.5% was obtained.
Keywords: gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement gold nanoparticles; magnetic nanoparticles; proteinase K; hydrolase; conductometric transducers; response enhancement
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Nouira, W.; Maaref, A.; Elaissari, H.; Vocanson, F.; Siadat, M.; Jaffrezic-Renault, N. Enhanced Response of a Proteinase K-Based Conductometric Biosensor Using Nanoparticles. Sensors 2014, 14, 13298-13307.

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