Sensors 2014, 14(7), 12958-12967; doi:10.3390/s140712958
Article

Short Chain N-Acyl Homoserine Lactone Production in Tropical Marine Vibrio sinaloensis Strain T47

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Received: 8 May 2014; in revised form: 30 June 2014 / Accepted: 11 July 2014 / Published: 18 July 2014
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Quorum sensing (QS), acts as one of the gene regulatory systems that allow bacteria to regulate their physiological activities by sensing the population density with synchronization of the signaling molecules that they produce. Here, we report a marine isolate, namely strain T47, and its unique AHL profile. Strain T47 was identified using 16S rRNA sequence analysis confirming that it is a member of Vibrio closely clustered to Vibrio sinaloensis. The isolated V. sinaloensis strain T47 was confirmed to produce N-butanoyl-L-homoserine lactone (C4-HSL) by using high resolution liquid chromatography tandem mass spectrometry. V. sinaloensis strain T47 also formed biofilms and its biofilm formation could be affected by anti-QS compound (cathechin) suggesting this is a QS-regulated trait in V. sinaloensis strain T47. To our knowledge, this is the first documentation of AHL and biofilm production in V. sinaloensis strain T47.
Keywords: 16S rRNA; biofilm; Chromobacterium violaceum CV026; marine seawater-borne bacteria; N-acylhomoserine lactone (AHL); N-butyryl-L-homoserine lactone (C4-HSL); quorum sensing (QS)
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MDPI and ACS Style

Tan, P.-W.; Tan, W.-S.; Yunos, N.Y.M.; Mohamad, N.I.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Short Chain N-Acyl Homoserine Lactone Production in Tropical Marine Vibrio sinaloensis Strain T47. Sensors 2014, 14, 12958-12967.

AMA Style

Tan P-W, Tan W-S, Yunos NYM, Mohamad NI, Adrian T-G-S, Yin W-F, Chan K-G. Short Chain N-Acyl Homoserine Lactone Production in Tropical Marine Vibrio sinaloensis Strain T47. Sensors. 2014; 14(7):12958-12967.

Chicago/Turabian Style

Tan, Pui-Wan; Tan, Wen-Si; Yunos, Nina Y.M.; Mohamad, Nur I.; Adrian, Tan-Guan-Sheng; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Short Chain N-Acyl Homoserine Lactone Production in Tropical Marine Vibrio sinaloensis Strain T47." Sensors 14, no. 7: 12958-12967.

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