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Sensors 2014, 14(7), 12511-12522; doi:10.3390/s140712511
Article

Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11

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1 Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia 2 Jeffrey Cheah School of Medicine & Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia
* Author to whom correspondence should be addressed.
Received: 7 May 2014 / Revised: 24 June 2014 / Accepted: 8 July 2014 / Published: 11 July 2014
(This article belongs to the Section Biosensors)
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Abstract

A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.
Keywords: MALDI-TOF; mass spectrometry; N-butyryl homoserine lactone (C4-HSL); N-hexanoyl homoserine lactone (C6-HSL); N-octanoyl homoserine lactone (C8-HSL); N-dodecanoyl homoserine lactone (C12-HSL); N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL); clinical pathogen; cell-to-cell communication MALDI-TOF; mass spectrometry; N-butyryl homoserine lactone (C4-HSL); N-hexanoyl homoserine lactone (C6-HSL); N-octanoyl homoserine lactone (C8-HSL); N-dodecanoyl homoserine lactone (C12-HSL); N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL); clinical pathogen; cell-to-cell communication
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Cheng, H.J.; Ee, R.; Cheong, Y.M.; Tan, W.-S.; Yin, W.-F.; Chan, K.-G. Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11. Sensors 2014, 14, 12511-12522.

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