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Sensors 2014, 14(7), 12511-12522; doi:10.3390/s140712511
Article

Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11

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Received: 7 May 2014 / Revised: 24 June 2014 / Accepted: 8 July 2014 / Published: 11 July 2014
(This article belongs to the Section Biosensors)
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Abstract

A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.
Keywords: MALDI-TOF; mass spectrometry; N-butyryl homoserine lactone (C4-HSL); N-hexanoyl homoserine lactone (C6-HSL); N-octanoyl homoserine lactone (C8-HSL); N-dodecanoyl homoserine lactone (C12-HSL); N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL); clinical pathogen; cell-to-cell communication MALDI-TOF; mass spectrometry; N-butyryl homoserine lactone (C4-HSL); N-hexanoyl homoserine lactone (C6-HSL); N-octanoyl homoserine lactone (C8-HSL); N-dodecanoyl homoserine lactone (C12-HSL); N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL); clinical pathogen; cell-to-cell communication
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cheng, H.J.; Ee, R.; Cheong, Y.M.; Tan, W.-S.; Yin, W.-F.; Chan, K.-G. Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11. Sensors 2014, 14, 12511-12522.

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