Abstract: A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.
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Cheng, H.J.; Ee, R.; Cheong, Y.M.; Tan, W.-S.; Yin, W.-F.; Chan, K.-G. Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11. Sensors 2014, 14, 12511-12522.
Cheng HJ, Ee R, Cheong YM, Tan W-S, Yin W-F, Chan K-G. Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11. Sensors. 2014; 14(7):12511-12522.
Cheng, Huey J.; Ee, Robson; Cheong, Yuet M.; Tan, Wen-Si; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11." Sensors 14, no. 7: 12511-12522.