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Sensors 2014, 14(7), 12104-12113; doi:10.3390/s140712104
Article

Characterisation of a Marine Bacterium Vibrio Brasiliensis T33 Producing N-acyl Homoserine Lactone Quorum Sensing Molecules

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Received: 30 March 2014; in revised form: 26 June 2014 / Accepted: 3 July 2014 / Published: 8 July 2014
(This article belongs to the Section Biosensors)
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Abstract: N-acylhomoserine lactones (AHL) plays roles as signal molecules in quorum sensing (QS) in most Gram-negative bacteria. QS regulates various physiological activities in relation with population density and concentration of signal molecules. With the aim of isolating marine water-borne bacteria that possess QS properties, we report here the preliminary screening of marine bacteria for AHL production using Chromobacterium violaceum CV026 as the AHL biosensor. Strain T33 was isolated based on preliminary AHL screening and further identified by using 16S rDNA sequence analysis as a member of the genus Vibrio closely related to Vibrio brasiliensis. The isolated Vibrio sp. strain T33 was confirmed to produce N-hexanoyl-l-homoserine lactone (C6-HSL) and N-(3-oxodecanoyl)-l-homoserine lactone (3-oxo-C10 HSL) through high resolution tandem mass spectrometry analysis. We demonstrated that this isolate formed biofilms which could be inhibited by catechin. To the best of our knowledge, this is the first report that documents the production of these AHLs by Vibrio brasiliensis strain T33.
Keywords: 16S rDNA; biosensor; Chromobacterium violaceum CV026; marine water-borne; N-acyl homoserine lactone (AHL); quorum sensing (QS); N-hexanoyl-l-homoserine lactone (C6-HSL); N-(3-oxo-decanoyl)-l-homoserine lactone (3-oxo-C10 HSL); Vibrio brasiliensis 16S rDNA; biosensor; Chromobacterium violaceum CV026; marine water-borne; N-acyl homoserine lactone (AHL); quorum sensing (QS); N-hexanoyl-l-homoserine lactone (C6-HSL); N-(3-oxo-decanoyl)-l-homoserine lactone (3-oxo-C10 HSL); Vibrio brasiliensis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tan, W.-S.; Yunos, N.Y.M.; Tan, P.-W.; Mohamad, N.I.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Characterisation of a Marine Bacterium Vibrio Brasiliensis T33 Producing N-acyl Homoserine Lactone Quorum Sensing Molecules. Sensors 2014, 14, 12104-12113.

AMA Style

Tan W-S, Yunos NYM, Tan P-W, Mohamad NI, Adrian T-G-S, Yin W-F, Chan K-G. Characterisation of a Marine Bacterium Vibrio Brasiliensis T33 Producing N-acyl Homoserine Lactone Quorum Sensing Molecules. Sensors. 2014; 14(7):12104-12113.

Chicago/Turabian Style

Tan, Wen-Si; Yunos, Nina Y.M.; Tan, Pui-Wan; Mohamad, Nur I.; Adrian, Tan-Guan-Sheng; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Characterisation of a Marine Bacterium Vibrio Brasiliensis T33 Producing N-acyl Homoserine Lactone Quorum Sensing Molecules." Sensors 14, no. 7: 12104-12113.


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