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Sensors 2014, 14(7), 11760-11769; doi:10.3390/s140711760
Article

Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity

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Received: 1 April 2014; in revised form: 21 June 2014 / Accepted: 26 June 2014 / Published: 3 July 2014
(This article belongs to the Section Biosensors)
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Abstract: We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
Keywords: Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ghani, N.A.; Norizan, S.N.M.; Chan, X.Y.; Yin, W.-F.; Chan, K.-G. Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity. Sensors 2014, 14, 11760-11769.

AMA Style

Ghani NA, Norizan SNM, Chan XY, Yin W-F, Chan K-G. Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity. Sensors. 2014; 14(7):11760-11769.

Chicago/Turabian Style

Ghani, Norshazliza A.; Norizan, Siti N.M.; Chan, Xin Y.; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity." Sensors 14, no. 7: 11760-11769.


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