Abstract: We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
Keywords: Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone
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Ghani, N.A.; Norizan, S.N.M.; Chan, X.Y.; Yin, W.-F.; Chan, K.-G. Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity. Sensors 2014, 14, 11760-11769.
Ghani NA, Norizan SNM, Chan XY, Yin W-F, Chan K-G. Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity. Sensors. 2014; 14(7):11760-11769.
Ghani, Norshazliza A.; Norizan, Siti N.M.; Chan, Xin Y.; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity." Sensors 14, no. 7: 11760-11769.