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Sensors 2014, 14(7), 11760-11769; doi:10.3390/s140711760
Article

Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity

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Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 1 April 2014 / Revised: 21 June 2014 / Accepted: 26 June 2014 / Published: 3 July 2014
(This article belongs to the Section Biosensors)
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Abstract

We report the degradation of quorum sensing N-acylhomoserine lactone molecules by a bacterium isolated from a Malaysian marine water sample. MALDI-TOF and phylogenetic analysis indicated this isolate BM1 clustered closely to Labrenzia sp. The quorum quenching activity of this isolate was confirmed by using a series of bioassays and rapid resolution liquid chromatography analysis. Labrenzia sp. degraded a wide range of N-acylhomoserine lactones namely N-(3-hexanoyl)-l-homoserine lactone (C6-HSL), N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-l-homoserine lactone (3-hydroxy-C6-HSL). Re-lactonisation bioassays confirmed Labrenzia sp. BM1 degraded these signalling molecules efficiently via lactonase activity. To the best of our knowledge, this is the first documentation of a Labrenzia sp. capable of degrading N-acylhomoserine lactones and confirmation of its lactonase-based mechanism of action.
Keywords: Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone Labrenzia sp.; quorum quenching; quorum sensing; marine; seawater; rapid resolution liquid chromatography (RRLC); N-acylhomoserine lactone; N-(3-oxohexanoyl)-l-homoserine lactone
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Ghani, N.A.; Norizan, S.N.M.; Chan, X.Y.; Yin, W.-F.; Chan, K.-G. Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity. Sensors 2014, 14, 11760-11769.

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