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Sensors 2014, 14(6), 10650-10663; doi:10.3390/s140610650

Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface

1
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
2
Institute of Biochemistry and Biophysics of Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland
3
Chemistry Department, University of Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium
*
Author to whom correspondence should be addressed.
Received: 8 April 2014 / Revised: 15 May 2014 / Accepted: 26 May 2014 / Published: 17 June 2014
(This article belongs to the Section Biosensors)
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Abstract

In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II) centres which were measured by Osteryoung square-wave voltammetry (OSWV). In order to verify whether the observed analytical signal originates from the recognition process between the His6–RAGE VC1 domains and the S100B protein, the electrode modified with the His6–RAGE C2 and His6–RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ1-40 have no influence on the biosensor performance. View Full-Text
Keywords: pentetic acid–Cu(II) complex; gold electrodes; His-tagged proteins; S100B protein; electrochemical biosensor pentetic acid–Cu(II) complex; gold electrodes; His-tagged proteins; S100B protein; electrochemical biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mikuła, E.; Wysłouch-Cieszyńska, A.; Zhukova, L.; Puchalska, M.; Verwilst, P.; Dehaen, W.; Radecki, J.; Radecka, H. Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface. Sensors 2014, 14, 10650-10663.

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