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Sensors 2014, 14(6), 10527-10537; doi:10.3390/s140610527
Article

Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties

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Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
* Author to whom correspondence should be addressed.
Received: 8 April 2014 / Revised: 4 June 2014 / Accepted: 11 June 2014 / Published: 13 June 2014
(This article belongs to the Section Biosensors)
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Abstract

One obvious requirement for concerted action by a bacterial population is for an individual to be aware of and respond to the other individuals of the same species in order to form a response in unison. The term “quorum sensing” (QS) was coined to describe bacterial communication that is able to stimulate expression of a series of genes when the concentration of the signaling molecules has reached a threshold level. Here we report the isolation from aquatic environment of a bacterium that was later identified as Enterobacter sp.. Chromobacterium violaceum CV026 and Escherichia coli [pSB401] were used for preliminary screening of N-acyl homoserine lactone (AHL) production. The Enterobacter sp. isolated was shown to produce two types of AHLs as confirmed by analysis using high resolution tandem mass spectrometry. To the best of our knowledge, this is the first documentation of an Enterobacter sp. that produced both 3-oxo-C6-HSL and 3-oxo-C8-HSL as QS signaling molecules.
Keywords: aquatic environment; Chromobacterium violaceum CV026; Escherichia coli [pSB401]; N-acyl homoserine lactones (AHLs); quorum sensing aquatic environment; Chromobacterium violaceum CV026; Escherichia coli [pSB401]; N-acyl homoserine lactones (AHLs); quorum sensing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tan, W.-S.; Yunos, N.Y.M.; Tan, P.-W.; Mohamad, N.I.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties. Sensors 2014, 14, 10527-10537.

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