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Sensors 2014, 14(6), 10203-10212; doi:10.3390/s140610203

Application of an Electrochemical Immunosensor with a MWCNT/PDAA Modified Electrode for Detection of Serum Trypsin

1
Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China
2
Department of Pathology, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China
3
Department of Laboratory Medicine, College of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350005, China
*
Author to whom correspondence should be addressed.
Received: 31 March 2014 / Revised: 11 May 2014 / Accepted: 16 May 2014 / Published: 10 June 2014
(This article belongs to the Section Biosensors)
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Abstract

Objective: To establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode. Method: A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Results: With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10–100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL. Conclusions: The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection. View Full-Text
Keywords: electrochemical immunosensor; trypsin; multiwalled carbon nanotubes; nanogold electrochemical immunosensor; trypsin; multiwalled carbon nanotubes; nanogold
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yi, Q.; Liu, Q.; Gao, F.; Chen, Q.; Wang, G. Application of an Electrochemical Immunosensor with a MWCNT/PDAA Modified Electrode for Detection of Serum Trypsin. Sensors 2014, 14, 10203-10212.

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