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Sensors 2014, 14(6), 10177-10186; doi:10.3390/s140610177
Article

Quorum Sensing Activity in Pandoraea pnomenusa RB38

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Received: 5 March 2014 / Revised: 25 May 2014 / Accepted: 28 May 2014 / Published: 10 June 2014
(This article belongs to the Section Biosensors)
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Abstract

Strain RB38 was recovered from a former dumping area in Malaysia. MALDI-TOF mass spectrometry and genomic analysis identified strain RB-38 as Pandoraea pnomenusa. Various biosensors confirmed its quorum sensing properties. High resolution triple quadrupole liquid chromatography–mass spectrometry analysis was subsequently used to characterize the N-acyl homoserine lactone production profile of P. pnomenusa strain RB38, which validated that this isolate produced N-octanoyl homoserine lactone as a quorum sensing molecule. This is the first report of the production of N-octanoyl homoserine lactone by P. pnomenusa strain RB38.
Keywords: matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF); mass spectrometry (MS); cell-to cell communication; triple quodruopole liquid chromatography mass spectrometry (LC-MS/MS); N-octanoyl homoserine lactone (C8-HSL) matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF); mass spectrometry (MS); cell-to cell communication; triple quodruopole liquid chromatography mass spectrometry (LC-MS/MS); N-octanoyl homoserine lactone (C8-HSL)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ee, R.; Lim, Y.-L.; Kin, L.-X.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity in Pandoraea pnomenusa RB38. Sensors 2014, 14, 10177-10186.

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