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Sensors 2014, 14(5), 8940-8949; doi:10.3390/s140508940
Article

Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries

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1 Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia 2 Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia
* Author to whom correspondence should be addressed.
Received: 18 April 2014 / Revised: 4 May 2014 / Accepted: 13 May 2014 / Published: 21 May 2014
(This article belongs to the Section Biosensors)
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Abstract

Bacteria realize the ability to communicate by production of quorum sensing (QS) molecules called autoinducers, which regulate the physiological activities in their ecological niches. The oral cavity could be a potential area for the presence of QS bacteria. In this study, we report the isolation of a QS bacterial isolate C10B from dentine caries. Preliminary screening using Chromobacterium violaceum CV026 biosensor showed that isolate C10B was able to produce N-acylhomoserine lactones (AHLs). This bacterium was further identified as a member of Burkholderia, an opportunistic pathogen. The isolated Burkholderia sp. was confirmed to produce N-hexanoyl-L-homoserine lactone (C6-HSL), N-octanoyl-L-homoserine lactone (C8-HSL), N-decanoyl-L-homoserine lactone (C10-HSL) and N-dodecanoyl-L-homoserine lactone (C12-HSL).
Keywords: Chromobacterium violaceum CV026; N-hexanoyl-L-homoserine lactone (C6-HSL); N-octanoyl-L-homoserine lactone (C8-HSL); N-decanoyl-L-homoserine lactone (C10-HSL); N-dodecanoyl-L-homoserine lactone (C12-HSL); oral cavity; dentine caries; quorum sensing (QS) Chromobacterium violaceum CV026; N-hexanoyl-L-homoserine lactone (C6-HSL); N-octanoyl-L-homoserine lactone (C8-HSL); N-decanoyl-L-homoserine lactone (C10-HSL); N-dodecanoyl-L-homoserine lactone (C12-HSL); oral cavity; dentine caries; quorum sensing (QS)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Goh, S.Y.; Tan, W.-S.; Khan, S.A.; Chew, H.P.; Kasim, N.H.A.; Yin, W.-F.; Chan, K.-G. Unusual Multiple Production of N-Acylhomoserine Lactones a by Burkholderia sp. Strain C10B Isolated from Dentine Caries. Sensors 2014, 14, 8940-8949.

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