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Sensors 2014, 14(5), 8305-8312; doi:10.3390/s140508305
Article

Quorum Sensing Activity of a Kluyvera sp. Isolated from a Malaysian Waterfall

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Received: 31 March 2014 / Revised: 30 April 2014 / Accepted: 4 May 2014 / Published: 8 May 2014
(This article belongs to the Section Biosensors)
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Abstract

In many species of bacteria, the quorum sensing mechanism is used as a unique communication system which allows them to regulate gene expression and behavior in accordance with their population density. N-Acylhomoserine lactones (AHLs) are known as diffusible autoinducer molecules involved in this communication network. This finding aimed to characterize the production of AHL of a bacterial strain ND04 isolated from a Malaysian waterfall. Strain ND04 was identified as Kluyvera sp. as confirmed by molecular analysis of its 16S ribosomal RNA gene sequence. Kluyvera sp. is closely related to the Enterobacteriaceae family. Chromobacterium violaceum CV026 was used as a biosensor to detect the production of AHL by strain ND04. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of strain ND04 showed our isolate produced two AHLs which are N-(3-oxohexanoyl)homoserine lactone (3-oxo-C6 HSL) and N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL).
Keywords: cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; N-(3-oxohexanoyl)homoserine lactone; N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL); Kluyvera sp. cell-to-cell communication; mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; N-(3-oxohexanoyl)homoserine lactone; N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8 HSL); Kluyvera sp.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yunos, N.Y.M.; Tan, W.-S.; Mohamad, N.I.; Tan, P.-W.; Adrian, T.-G.-S.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of a Kluyvera sp. Isolated from a Malaysian Waterfall. Sensors 2014, 14, 8305-8312.

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