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Sensors 2014, 14(4), 6463-6473; doi:10.3390/s140406463

Rhodotorula Mucilaginosa, a Quorum Quenching Yeast Exhibiting Lactonase Activity Isolated from a Tropical Shoreline

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
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Received: 26 January 2014 / Revised: 19 March 2014 / Accepted: 21 March 2014 / Published: 9 April 2014
(This article belongs to the Section Biosensors)
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Abstract

Two microbial isolates from a Malaysian shoreline were found to be capable of degrading N-acylhomoserine lactones. Both Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry and 18S rDNA phylogenetic analyses confirmed that these isolates are Rhodotorula mucilaginosa. Quorum quenching activities were detected by a series of bioassays and rapid resolution liquid chromatography analysis. The isolates were able to degrade various quorum sensing molecules namely N-hexanoyl-L-homoserine lactone (C6-HSL), N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL). Using a relactonisation assay to verify the quorum quenching mechanism, it is confirmed that Rh. mucilaginosa degrades the quorum sensing molecules via lactonase activity. To the best of our knowledge, this is the first documentation of the fact that Rh. mucilaginosa has activity against a broad range of AHLs namely C6-HSL, 3-oxo-C6-HSL and 3-hydroxy-C6-HSL. View Full-Text
Keywords: N-hexanoyl-L-homoserine lactone (C6-HSL); N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL); N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL); quorum sensing; quorum quenching N-hexanoyl-L-homoserine lactone (C6-HSL); N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL); N-(3-hydroxyhexanoyl)-L-homoserine lactone (3-hydroxy-C6-HSL); quorum sensing; quorum quenching
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MDPI and ACS Style

Ghani, N.A.; Sulaiman, J.; Ismail, Z.; Chan, X.-Y.; Yin, W.-F.; Chan, K.-G. Rhodotorula Mucilaginosa, a Quorum Quenching Yeast Exhibiting Lactonase Activity Isolated from a Tropical Shoreline. Sensors 2014, 14, 6463-6473.

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