Sensors 2014, 14(3), 5136-5146; doi:10.3390/s140305136
Article

Quorum Sensing Activity of Serratia fonticola Strain RB-25 Isolated from an Ex-landfill Site

1email, 1email, 2email, 1email and 1,* email
Received: 16 January 2014; in revised form: 17 February 2014 / Accepted: 26 February 2014 / Published: 12 March 2014
(This article belongs to the Section Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Quorum sensing is a unique bacterial communication system which permits bacteria to synchronize their behaviour in accordance with the population density. The operation of this communication network involves the use of diffusible autoinducer molecules, termed N-acylhomoserine lactones (AHLs). Serratia spp. are well known for their use of quorum sensing to regulate the expression of various genes. In this study, we aimed to characterized the AHL production of a bacterium designated as strain RB-25 isolated from a former domestic waste landfill site. It was identified as Serratia fonticola using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry analysis and this was confirmed by 16S ribosomal DNA sequencing. High resolution triple quadrupole liquid chromatography-mass spectrometry analysis of S. fonticola strain RB-25 spent culture supernatant indicated the existence of three AHLs namely: N-butyryl-L-homoserine lactone (C4-HSL), N-hexanoyl-L-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6 HSL). This is the first report of the production of these AHLs in S. fonticola.
Keywords: cell-to-cell communication; matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF); mass spectrometry; triple quadrupole liquid chromatography mass spectrometry; N-butyryl-L-homoserine lactone (C4-HSL); N-hexanoyl-L-homoserine lactone (C6-HSL); N-(3-oxohexanoyl) homoserine-lactone (3-oxo-C6 HSL)
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MDPI and ACS Style

Ee, R.; Lim, Y.-L.; Tee, K.-K.; Yin, W.-F.; Chan, K.-G. Quorum Sensing Activity of Serratia fonticola Strain RB-25 Isolated from an Ex-landfill Site. Sensors 2014, 14, 5136-5146.

AMA Style

Ee R, Lim Y-L, Tee K-K, Yin W-F, Chan K-G. Quorum Sensing Activity of Serratia fonticola Strain RB-25 Isolated from an Ex-landfill Site. Sensors. 2014; 14(3):5136-5146.

Chicago/Turabian Style

Ee, Robson; Lim, Yan-Lue; Tee, Kok-Keng; Yin, Wai-Fong; Chan, Kok-Gan. 2014. "Quorum Sensing Activity of Serratia fonticola Strain RB-25 Isolated from an Ex-landfill Site." Sensors 14, no. 3: 5136-5146.

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