Abstract: In this article we report a new, simple, and reliable optical read-out detection method able to assess Rotavirus present in human sera as well as in the viral pollution sources. It is based on the interference of two interferometers used as biophotonic transducers. The method significantly improves the optical label-free biosensing response measuring both, the concentration of the AgR and its corresponding size. Two different immunoassays were carried out: Bovine Serum Albumin (BSA), and the recognition by its antibody (anti-BSA); and Rotavirus (AgR) and the recognition by its antibody (anti-AgR). In the cases studied, and using as model interferometer a simple Fabry-Perot transducer, we demonstrate a biosensing enhancement of two orders of magnitude in the Limit of Detection (LoD). In fact, this read-out optical method may have significant implications to enhance other optical label-free photonic transducers reported in the scientific literature.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Holgado, M.; Sanza, F.J.; López, A.; Lavín, Á.; Casquel, R.; Laguna, M.F. Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species. Sensors 2014, 14, 3675-3689.
Holgado M, Sanza FJ, López A, Lavín Á, Casquel R, Laguna MF. Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species. Sensors. 2014; 14(2):3675-3689.
Holgado, Miguel; Sanza, Francisco J.; López, Ana; Lavín, Álvaro; Casquel, Rafael; Laguna, María F. 2014. "Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species." Sensors 14, no. 2: 3675-3689.