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Sensors 2014, 14(2), 3675-3689; doi:10.3390/s140203675

Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species

1
Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain
2
Applied Physics Department, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal 2, Madrid 28006, Spain
*
Author to whom correspondence should be addressed.
Received: 16 December 2013 / Revised: 23 January 2014 / Accepted: 19 February 2014 / Published: 21 February 2014
(This article belongs to the Special Issue Photonic Sensors for Industrial, Environmental and Health Monitoring)
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Abstract

In this article we report a new, simple, and reliable optical read-out detection method able to assess Rotavirus present in human sera as well as in the viral pollution sources. It is based on the interference of two interferometers used as biophotonic transducers. The method significantly improves the optical label-free biosensing response measuring both, the concentration of the AgR and its corresponding size. Two different immunoassays were carried out: Bovine Serum Albumin (BSA), and the recognition by its antibody (anti-BSA); and Rotavirus (AgR) and the recognition by its antibody (anti-AgR). In the cases studied, and using as model interferometer a simple Fabry-Perot transducer, we demonstrate a biosensing enhancement of two orders of magnitude in the Limit of Detection (LoD). In fact, this read-out optical method may have significant implications to enhance other optical label-free photonic transducers reported in the scientific literature. View Full-Text
Keywords: photonic biosensors; label-free; rotavirus; optical read-out; environmental monitoring; health monitoring photonic biosensors; label-free; rotavirus; optical read-out; environmental monitoring; health monitoring
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Holgado, M.; Sanza, F.J.; López, A.; Lavín, Á.; Casquel, R.; Laguna, M.F. Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species. Sensors 2014, 14, 3675-3689.

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