Next Article in Journal
Performance Improvement in Geographic Routing for Vehicular Ad Hoc Networks
Previous Article in Journal
Efficient High-Rate Satellite Clock Estimation for PPP Ambiguity Resolution Using Carrier-Ranges
Article Menu

Export Article

Open AccessReview
Sensors 2014, 14(12), 22313-22341; doi:10.3390/s141222313

Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical Use

Division of Biology, Chemistry, and Materials Science Office of Science and Engineering Laboratories; U.S. Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA
*
Author to whom correspondence should be addressed.
Received: 19 August 2014 / Revised: 12 November 2014 / Accepted: 13 November 2014 / Published: 25 November 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1482 KB, uploaded 25 November 2014]   |  

Abstract

This review investigates optical sensor platforms for protein multiplexing, the ability to analyze multiple analytes simultaneously. Multiplexing is becoming increasingly important for clinical needs because disease and therapeutic response often involve the interplay between a variety of complex biological networks encompassing multiple, rather than single, proteins. Multiplexing is generally achieved through one of two routes, either through spatial separation on a surface (different wells or spots) or with the use of unique identifiers/labels (such as spectral separation—different colored dyes, or unique beads—size or color). The strengths and weaknesses of conventional platforms such as immunoassays and new platforms involving protein arrays and lab-on-a-chip technology, including commercially-available devices, are discussed. Three major public health concerns are identified whereby detecting medically-relevant markers using Point-of-Care (POC) multiplex assays could potentially allow for a more efficient diagnosis and treatment of diseases. View Full-Text
Keywords: immunoassay; fluorescent; ELISA; multiplex; protein; biomarker; point-of-care; in vitro diagnostics; quantum dot immunoassay; fluorescent; ELISA; multiplex; protein; biomarker; point-of-care; in vitro diagnostics; quantum dot
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Spindel, S.; Sapsford, K.E. Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical Use. Sensors 2014, 14, 22313-22341.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top