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Sensors 2014, 14(11), 22128-22139; doi:10.3390/s141122128

Novel Membrane-Based Electrochemical Sensor for Real-Time Bio-Applications

Department of Micro- and Nanotechnology, Technical University of Denmark, ├śrsteds Plads, 2800 Kgs. Lyngby, Denmark
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Received: 30 July 2014 / Revised: 4 November 2014 / Accepted: 14 November 2014 / Published: 24 November 2014
(This article belongs to the Section Biosensors)
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Abstract

This article presents a novel membrane-based sensor for real-time electrochemical investigations of cellular- or tissue cultures. The membrane sensor enables recording of electrical signals from a cell culture without any signal dilution, thus avoiding loss of sensitivity. Moreover, the porosity of the membrane provides optimal culturing conditions similar to existing culturing techniques allowing more efficient nutrient uptake and molecule release. The patterned sensor electrodes were fabricated on a porous membrane by electron-beam evaporation. The electrochemical performance of the membrane electrodes was characterized by cyclic voltammetry and chronoamperometry, and the detection of synthetic dopamine was demonstrated down to a concentration of 3.1 pM. Furthermore, to present the membrane-sensor functionality the dopamine release from cultured PC12 cells was successfully measured. The PC12 cells culturing experiments showed that the membrane-sensor was suitable as a cell culturing substrate for bio-applications. Real-time measurements of dopamine exocytosis in cell cultures were performed, where the transmitter release was recorded at the point of release. The developed membrane-sensor provides a new functionality to the standard culturing methods, enabling sensitive continuous in vitro monitoring and closely mimicking the in vivo conditions. View Full-Text
Keywords: membrane electrodes; electrochemical sensing; membrane-sensor; real-time monitoring; dopamine; PC12 cells membrane electrodes; electrochemical sensing; membrane-sensor; real-time monitoring; dopamine; PC12 cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Alatraktchi, F.A.; Bakmand, T.; Dimaki, M.; Svendsen, W.E. Novel Membrane-Based Electrochemical Sensor for Real-Time Bio-Applications. Sensors 2014, 14, 22128-22139.

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