Next Article in Journal
Chaotic Extension Neural Network Theory-Based XXY Stage Collision Fault Detection Using a Single Accelerometer Sensor
Next Article in Special Issue
Rapid Immunoenzyme Assay of Aflatoxin B1 Using Magnetic Nanoparticles
Previous Article in Journal
Electrically Insulated Sensing of Respiratory Rate and Heartbeat Using Optical Fibers
Previous Article in Special Issue
Using Bio-Functionalized Magnetic Nanoparticles and Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive Bio-Detection
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(11), 21535-21548; doi:10.3390/s141121535

Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles

Institute of Hydrochemistry and Chemical Balneology, Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, Munich 81377, Germany
*
Author to whom correspondence should be addressed.
Received: 11 October 2014 / Revised: 7 November 2014 / Accepted: 11 November 2014 / Published: 14 November 2014
(This article belongs to the Special Issue Nanoparticle-Based Biosensors)
View Full-Text   |   Download PDF [901 KB, uploaded 14 November 2014]   |  

Abstract

A competitive colorimetric immunoassay for the detection of aflatoxin B1 (AFB) has been established using biofunctionalized magnetic beads (MBs) and gold nanoparticles (GNPs). Aflatoxin B1-bovine serum albumin conjugates (AFB-BSA) modified MBs were employed as capture probe, which could specifically bind with GNP-labeled anti-AFB antibodies through immunoreaction, while such specific binding was competitively inhibited by the addition of AFB. After magnetic separation, the supernatant solution containing unbound GNPs was directly tested by UV-Vis spectroscopy. The absorption intensity was directly proportional to the AFB concentration. The influence of GNP size, incubation time and pH was investigated in detail. After optimization, the developed method could detect AFB in a linear range from 20 to 800 ng/L, with the limit of detection at 12 ng/L. The recoveries for spiked maize samples ranged from 92.8% to 122.0%. The proposed immunoassay provides a promising approach for simple, rapid, specific and cost-effective detection of toxins in the field of food safety. View Full-Text
Keywords: aflatoxin B1; magnetic beads; gold nanoparticles; colorimetric detection; competitive immunoassay aflatoxin B1; magnetic beads; gold nanoparticles; colorimetric detection; competitive immunoassay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, X.; Niessner, R.; Knopp, D. Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles. Sensors 2014, 14, 21535-21548.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top