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Sensors 2014, 14(11), 21535-21548; doi:10.3390/s141121535

Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles

Institute of Hydrochemistry and Chemical Balneology, Chair for Analytical Chemistry, Technische Universität München, Marchioninistrasse 17, Munich 81377, Germany
Author to whom correspondence should be addressed.
Received: 11 October 2014 / Revised: 7 November 2014 / Accepted: 11 November 2014 / Published: 14 November 2014
(This article belongs to the Special Issue Nanoparticle-Based Biosensors)
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A competitive colorimetric immunoassay for the detection of aflatoxin B1 (AFB) has been established using biofunctionalized magnetic beads (MBs) and gold nanoparticles (GNPs). Aflatoxin B1-bovine serum albumin conjugates (AFB-BSA) modified MBs were employed as capture probe, which could specifically bind with GNP-labeled anti-AFB antibodies through immunoreaction, while such specific binding was competitively inhibited by the addition of AFB. After magnetic separation, the supernatant solution containing unbound GNPs was directly tested by UV-Vis spectroscopy. The absorption intensity was directly proportional to the AFB concentration. The influence of GNP size, incubation time and pH was investigated in detail. After optimization, the developed method could detect AFB in a linear range from 20 to 800 ng/L, with the limit of detection at 12 ng/L. The recoveries for spiked maize samples ranged from 92.8% to 122.0%. The proposed immunoassay provides a promising approach for simple, rapid, specific and cost-effective detection of toxins in the field of food safety. View Full-Text
Keywords: aflatoxin B1; magnetic beads; gold nanoparticles; colorimetric detection; competitive immunoassay aflatoxin B1; magnetic beads; gold nanoparticles; colorimetric detection; competitive immunoassay

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, X.; Niessner, R.; Knopp, D. Magnetic Bead-Based Colorimetric Immunoassay for Aflatoxin B1 Using Gold Nanoparticles. Sensors 2014, 14, 21535-21548.

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