Next Article in Journal
The MAIN Shirt: A Textile-Integrated Magnetic Induction Sensor Array
Previous Article in Journal
Managing the Number of Tag Bits Transmitted in a Bit-Tracking RFID Collision Resolution Protocol
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(1), 1028-1038; doi:10.3390/s140101028

Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis

Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania
*
Author to whom correspondence should be addressed.
Received: 6 November 2013 / Revised: 16 December 2013 / Accepted: 24 December 2013 / Published: 9 January 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [254 KB, uploaded 21 June 2014]   |  

Abstract

A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h−1 with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 µM for methanol and 0.2 µM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement.
Keywords: alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Radulescu, M.-C.; Bucur, B.; Bucur, M.-P.; Radu, G.L. Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis. Sensors 2014, 14, 1028-1038.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top