Next Article in Journal
The MAIN Shirt: A Textile-Integrated Magnetic Induction Sensor Array
Previous Article in Journal
Managing the Number of Tag Bits Transmitted in a Bit-Tracking RFID Collision Resolution Protocol
Sensors 2014, 14(1), 1028-1038; doi:10.3390/s140101028
Article

Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis

* ,
,
 and
Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 296, Splaiul Independentei, Bucharest 060031, Romania
* Author to whom correspondence should be addressed.
Received: 6 November 2013 / Revised: 16 December 2013 / Accepted: 24 December 2013 / Published: 9 January 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [254 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h−1 with an RSD of 2.7%. The detection limits for both batch and FIA measurements were 0.1 µM for methanol and 0.2 µM for aspartame, respectively. The enzymatic biosensor was successfully applied for aspartame determination in different sample matrices/commercial products (liquid and solid samples) without any pre-treatment step prior to measurement.
Keywords: alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame alcohol oxidase; carboxyl esterase; flow injection analysis; aspartame
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Radulescu, M.-C.; Bucur, B.; Bucur, M.-P.; Radu, G.L. Bienzymatic Biosensor for Rapid Detection of Aspartame by Flow Injection Analysis. Sensors 2014, 14, 1028-1038.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert