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Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization
Medical Informatics and Biological Micro-Electro-Mechanical Systems (MIMEMS) Specialized Laboratory, Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
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Received: 7 May 2013; in revised form: 30 May 2013 / Accepted: 14 June 2013 / Published: 12 July 2013
Abstract: During the last three decades; dielectrophoresis (DEP) has become a vital tool for cell manipulation and characterization due to its non-invasiveness. It is very useful in the trend towards point-of-care systems. Currently, most efforts are focused on using DEP in biomedical applications, such as the spatial manipulation of cells, the selective separation or enrichment of target cells, high-throughput molecular screening, biosensors and immunoassays. A significant amount of research on DEP has produced a wide range of microelectrode configurations. In this paper; we describe the microarray dot electrode, a promising electrode geometry to characterize and manipulate cells via DEP. The advantages offered by this type of microelectrode are also reviewed. The protocol for fabricating planar microelectrodes using photolithography is documented to demonstrate the fast and cost-effective fabrication process. Additionally; different state-of-the-art Lab-on-a-Chip (LOC) devices that have been proposed for DEP applications in the literature are reviewed. We also present our recently designed LOC device, which uses an improved microarray dot electrode configuration to address the challenges facing other devices. This type of LOC system has the capability to boost the implementation of DEP technology in practical settings such as clinical cell sorting, infection diagnosis, and enrichment of particle populations for drug development.
Keywords: dielectrophoresis; Lab-on-a-Chip; dot electrode; BioMEMS; particle manipulation
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Yafouz, B.; Kadri, N.A.; Ibrahim, F. Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization. Sensors 2013, 13, 9029-9046.
Yafouz B, Kadri NA, Ibrahim F. Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization. Sensors. 2013; 13(7):9029-9046.
Yafouz, Bashar; Kadri, Nahrizul A.; Ibrahim, Fatimah. 2013. "Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization." Sensors 13, no. 7: 9029-9046.