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Sensors 2013, 13(5), 5749-5756; doi:10.3390/s130505749
Article

Dual-Specific Interaction to Detect DNA on Gold Nanoparticles

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Received: 11 March 2013; in revised form: 1 April 2013 / Accepted: 7 April 2013 / Published: 3 May 2013
(This article belongs to the Section Biosensors)
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Abstract: An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
Keywords: nanoparticles; fluorophore dye; quenching; streptavidin/biotin; DNA hybridization; sensors nanoparticles; fluorophore dye; quenching; streptavidin/biotin; DNA hybridization; sensors
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Feng, C.-L.; Dou, X.-Q.; Liu, Q.-L.; Zhang, W.; Gu, J.-J.; Zhu, S.-M.; Jenkins, A.T.A.; Zhang, D. Dual-Specific Interaction to Detect DNA on Gold Nanoparticles. Sensors 2013, 13, 5749-5756.

AMA Style

Feng C-L, Dou X-Q, Liu Q-L, Zhang W, Gu J-J, Zhu S-M, Jenkins ATA, Zhang D. Dual-Specific Interaction to Detect DNA on Gold Nanoparticles. Sensors. 2013; 13(5):5749-5756.

Chicago/Turabian Style

Feng, Chuan-Liang; Dou, Xiao-Qiu; Liu, Qing-Lei; Zhang, Wang; Gu, Jia-Jun; Zhu, Shen-Min; Jenkins, Andrew T.A.; Zhang, Di. 2013. "Dual-Specific Interaction to Detect DNA on Gold Nanoparticles." Sensors 13, no. 5: 5749-5756.


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