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Dual-Specific Interaction to Detect DNA on Gold Nanoparticles
State Key Lab of Metal Matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China
Department of Chemistry, University of Bath, Bath BA2 7AY, UK
* Author to whom correspondence should be addressed.
Received: 11 March 2013; in revised form: 1 April 2013 / Accepted: 7 April 2013 / Published: 3 May 2013
Abstract: An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
Keywords: nanoparticles; fluorophore dye; quenching; streptavidin/biotin; DNA hybridization; sensors
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Feng, C.-L.; Dou, X.-Q.; Liu, Q.-L.; Zhang, W.; Gu, J.-J.; Zhu, S.-M.; Jenkins, A.T.A.; Zhang, D. Dual-Specific Interaction to Detect DNA on Gold Nanoparticles. Sensors 2013, 13, 5749-5756.
Feng C-L, Dou X-Q, Liu Q-L, Zhang W, Gu J-J, Zhu S-M, Jenkins ATA, Zhang D. Dual-Specific Interaction to Detect DNA on Gold Nanoparticles. Sensors. 2013; 13(5):5749-5756.
Feng, Chuan-Liang; Dou, Xiao-Qiu; Liu, Qing-Lei; Zhang, Wang; Gu, Jia-Jun; Zhu, Shen-Min; Jenkins, Andrew T.A.; Zhang, Di. 2013. "Dual-Specific Interaction to Detect DNA on Gold Nanoparticles." Sensors 13, no. 5: 5749-5756.